Department of Urology, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China.
Department of Urology, Guanxian Center Hospital, Liaocheng 252500, Shandong, China.
Biosci Rep. 2017 Nov 15;37(6). doi: 10.1042/BSR20171082. Print 2017 Dec 22.
The purpose of the study is to investigate the correlation between the expression of C-reactive protein () and autophagy-related 9B () and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of and on the apoptosis and cell cycle progression of CCRCC cell line. expression in CCRCC tissues and adjacent renal tissues was analyzed by immunohistochemistry (IHC). Gene expression was determined at transcription and translational levels using real-time quantitative PCR (RT-qPCR) and Western blot. The association between expression and clinical-pathological parameters including age, gender, pathological grades, TNM stage and distant metastasis of the patients was assessed by correlation analysis. siRNA and overexpression plasmids construction were used to manipulate the expression of in human CCRCC cell line 786-O. Cell apoptosis and cell cycle progression were determined using flow cytometry (FCM) and Hoechst 33258 staining. expression correlates with expression. The expression of and are significantly correlated with TNM staging, distant metastasis, and survival time of CCRCC patients. A high-level of indicates a poor overall survival (OS). In addition, expression influences cell cycle and apoptosis of CCRCC cells. The study reveals that might be a CCRCC development promoter. In addition, there is a close relationship between and in CCRCC carcinogenesis.
本研究旨在探讨 C 反应蛋白（）与自噬相关蛋白 9B（）的表达与透明细胞肾细胞癌（CCRCC）患者病理特征之间的相关性。我们还旨在探讨操纵和表达对 CCRCC 细胞系凋亡和细胞周期进程的影响。采用免疫组织化学（IHC）分析 CCRCC 组织和相邻肾组织中的表达。采用实时定量 PCR（RT-qPCR）和 Western blot 检测基因在转录和翻译水平上的表达。通过相关性分析评估表达与患者年龄、性别、病理分级、TNM 分期和远处转移等临床病理参数之间的关系。采用 siRNA 和过表达质粒构建来操纵人 CCRCC 细胞系 786-O 中的表达。采用流式细胞术（FCM）和 Hoechst 33258 染色测定细胞凋亡和细胞周期进程。表达与表达相关。和的表达与 TNM 分期、远处转移和 CCRCC 患者的生存时间显著相关。高水平的预示着总体生存率（OS）较差。此外，表达影响 CCRCC 细胞的细胞周期和凋亡。本研究表明可能是 CCRCC 发展的促进剂。此外，在 CCRCC 发生癌变过程中与之间存在密切关系。
