Emami Seyed Ahmad, Sadeghi-Aliabadi Hojjat, Saeidi Morteza, Jafarian Abbas
a Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran.
b Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Pharm Biol. 2005;43(4):299-304. doi: 10.1080/13880200590951676.
In this study, branchlets, fruit, or bark of Taxus baccata. L. as well as branchlets or fruits of two other species of Iranian conifers, namely, Platycladus orientalis. France and Cupressus sempervirens. L. var. horizentalis. (Mill) Gunde were collected, identified, and the cytotoxic effects of hydroalcoholic extracts on three human tumor cell lines were determined. Different concentrations of extracts were added to cultured cells and incubated for 72 h. Cell survival was evaluated using the MTT assay. Extracts from bark of female Taxus baccata. showed inhibitory activities against Hela cells. The extracts of the branchlets of male and female T. baccata. as well as obtained extract from fruits of P. orientalis. showed inhibitory activities against MDA-MB-468 cells, whereas the extracts of branchlets of female T. baccata. showed inhibitory activities against KB cells. In conclusion, obtained extract from bark of T. baccata. showed comparable cytotoxic effect to doxorubicin against Hela cells.
在本研究中，采集了欧洲红豆杉的小枝、果实或树皮，以及另外两种伊朗针叶树即东方扁柏和地中海柏木的小枝或果实，进行了鉴定，并测定了水醇提取物对三种人类肿瘤细胞系的细胞毒性作用。将不同浓度的提取物添加到培养细胞中并孵育72小时。使用MTT法评估细胞存活率。欧洲红豆杉雌株树皮提取物对宫颈癌细胞表现出抑制活性。欧洲红豆杉雄株和雌株小枝提取物以及东方扁柏果实提取物对MDA-MB-468细胞表现出抑制活性，而欧洲红豆杉雌株小枝提取物对KB细胞表现出抑制活性。总之，欧洲红豆杉树皮提取物对宫颈癌细胞表现出与阿霉素相当的细胞毒性作用。
