Shokrzadeh M, Azadbakht M, Ahangar N, Naderi H, Saeedi Saravi S S
Department of Toxicology-Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Mazandaran Pharmaceutical Sciences Research Center, Iran.
Pharmacogn Mag. 2010 Apr;6(22):102-5. doi: 10.4103/0973-1296.62894. Epub 2010 May 5.
Isolation and identification of some potent anti-tumor compounds from medicinal plants has motivated researchers to screen different parts of plant species for the determination of anti-tumor effects. In this study, cytotoxic effects and IC(50) of specific concentrations of hydro-alcoholic extracts of fruits of Juniperus sabina and leaves of Zataria multiflora were compared with hydro-alcoholic extract of bark of Taxus baccata and Cisplatin, well-known anticancer compounds, on normal (CHO and rat fibroblast) and cancer (HepG2 and SKOV3) cell lines. The hydro-alcoholic extracts of the plants were prepared by percolation. The cytotoxic effects and IC(50) of the extracts on the cell lines were studied followed by colonogenic assay after 72 h incubation. The results showed that the extract of Juniperus sabina possesses lower IC(50) in comparison with Zataria multiflora extract on all 4 normal and cancer cell lines (P<0.05); but, IC(50) of the Juniperus sabina extract was significantly higher than the Taxus baccata extract and Cisplatin on all 4 normal and cancer cell lines (P<0.05). As a result, it is concluded that the extract of J. sabina has almost similar cytotoxicity with the extract of Taxus baccata on cancer cells.
从药用植物中分离和鉴定一些有效的抗肿瘤化合物，促使研究人员筛选植物物种的不同部位以确定其抗肿瘤作用。在本研究中，将杜松果实和多花百里香叶的水醇提取物特定浓度的细胞毒性作用和IC50，与欧洲红豆杉树皮的水醇提取物和顺铂（著名的抗癌化合物）对正常（CHO和大鼠成纤维细胞）和癌症（HepG2和SKOV3）细胞系的作用进行了比较。植物的水醇提取物通过渗漉法制备。在72小时孵育后，通过集落形成试验研究提取物对细胞系的细胞毒性作用和IC50。结果表明，在所有4种正常和癌细胞系上，杜松提取物的IC50低于多花百里香提取物（P<0.05）；但是，在所有4种正常和癌细胞系上，杜松提取物的IC50显著高于欧洲红豆杉提取物和顺铂（P<0.05）。因此，得出结论，杜松提取物在癌细胞上具有与欧洲红豆杉提取物几乎相似的细胞毒性。
