School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600 UKM, Selangor, Malaysia.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Perlis, Malaysia.
Biosensors (Basel). 2017 Sep 18;7(3):38. doi: 10.3390/bios7030038.
An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO₂) particles for hydrogen peroxide (H₂O₂) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO₂, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H₂O₂ in solution. The analysis was carried out using an alternative impedance method, in which the biosensor exhibited a wide linear range response between 1 × 10 M and 1.5 × 10 M and a limit of detection (LOD) of 1 × 10 M without a redox mediator.
本研究的主要特点是构建了一种基于金纳米粒子（AuNP）的阻抗生物传感器，该生物传感器被二氧化钛（TiO₂）颗粒捕获，用于检测过氧化氢（H₂O₂）。生物传感器的基质采用 TiO₂表面，该表面先前使用 3-氨丙基三乙氧基硅烷（APTS）在低温下用胺末端基团修饰，以形成适合生物传感器应用的固定化表面。血红蛋白（Hb）作为生物传感器中的生物受体，用于检测溶液中的 H₂O₂。分析采用替代阻抗法进行，生物传感器在 1×10 M 和 1.5×10 M 之间表现出宽线性范围响应，并且在没有氧化还原介体的情况下检测限（LOD）为 1×10 M。
