Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, China.
Institute of Solid State Physics, Shanxi Datong University, Datong 037009, China.
Biosensors (Basel). 2020 Mar 16;10(3):25. doi: 10.3390/bios10030025.
A novel recyclable surface-enhanced Raman scattering (SERS)-based immunoassay was demonstrated and exhibited extremely high sensitivity toward prostate specific antigen (PSA). The immunoassay, which possessed a sandwich structure, was constructed of multifunctional FeO@TiO@Au nanocomposites as immune probe and Ag-coated sandpaper as immune substrate. First, by adjusting the density of outside Au seeds on FeO@TiO core-shell nanoparticles (NPs), the structure-dependent SERS and photocatalytic performance of the samples was explored by monitoring and degradating 4-mercaptobenzonic acid (4MBA). Afterwards, the SERS enhancement capability of Ag-coated sandpaper with different meshes was investigated, and a limit of detection (LOD), as low as 0.014 mM, was achieved by utilizing the substrate. Subsequently, the recyclable feasibility of PSA detection was approved by zeta potential measurement, absorption spectra, and SEM images and, particularly, more than 80% of SERS intensity still existed after even six cycles of immunoassay. The ultralow LOD of the recyclable immunoassay was finally calculated to be 1.871 pg/mL. Therefore, the recyclable SERS-based immunoassay exhibits good application prospects for diagnosis of cancer in clinical measurements.
一种新型的可回收表面增强拉曼散射（SERS）免疫分析方法被提出，并对前列腺特异性抗原（PSA）表现出极高的灵敏度。该免疫分析方法具有三明治结构，由多功能 FeO@TiO@Au 纳米复合材料作为免疫探针和镀银砂纸作为免疫基底构建而成。首先，通过调整 FeO@TiO 核壳纳米粒子（NPs）外表面 Au 种子的密度，通过监测和降解 4-巯基苯甲酸（4MBA）来探索样品的结构依赖性 SERS 和光催化性能。随后，研究了不同目数镀银砂纸的 SERS 增强能力，并利用该基底实现了低至 0.014 mM 的检测限（LOD）。随后，通过zeta 电位测量、吸收光谱和 SEM 图像证实了 PSA 检测的可回收可行性，即使经过六次免疫分析循环，SERS 强度仍存在 80%以上。最终计算出可回收 SERS 免疫分析的超低 LOD 为 1.871 pg/mL。因此，这种可回收的 SERS 免疫分析在临床测量中用于癌症诊断具有良好的应用前景。
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