Rajendran Vijayakumar, Krishnegowda Anilkumar, Nachiappan Vasanthi
Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 6200 24 India.
Department of Spice and Flavour Sciences, Central Food Technological Research Institute, Mysore, 570 020 India.
J Food Sci Technol. 2017 Aug;54(9):2965-2972. doi: 10.1007/s13197-017-2735-0. Epub 2017 Jun 6.
The herb has been traditionally used in India for medicinal purposes to treat hyperglycemia, diabetes, rheumatism, asthma, and skin diseases. In the present study, ethanolic extract of flower (Et-CAF) depicted anti-hyperlipidemic effect in the budding yeast cells. The hyperlipidemic conditions were induced in the yeast cells with oleic acid which showed an increase in triacylglycerol (TAG) and sterol esters (SE), and was supported by the mRNA expression of LRO1 and DGA1 (involved in TAG formation); as well as ARE1 and ARE2 (involved in SE formation). The anti-hyperlipidemic effect by the Et-CAF was compared with the commercial drug Atorvastatin. The lipid droplets were increased in the hyperlipidemic yeast cell that was observed under the confocal microscope with BODIPY staining; Atorvastatin and Et-CAF reduced the lipid droplets. This study revealed that the anti-hyperlipidemic effect in Et-CAF has gained importance and might be used to fill the gap created by the allopathic drugs.
这种草药在印度传统上被用于治疗高血糖、糖尿病、风湿病、哮喘和皮肤病等药用目的。在本研究中，花的乙醇提取物（Et-CAF）在出芽酵母细胞中表现出抗高血脂作用。用油酸在酵母细胞中诱导高血脂状态，这导致三酰甘油（TAG）和甾醇酯（SE）增加，并得到参与TAG形成的LRO1和DGA1以及参与SE形成的ARE1和ARE2的mRNA表达的支持。将Et-CAF的抗高血脂作用与商业药物阿托伐他汀进行比较。通过BODIPY染色在共聚焦显微镜下观察到，高血脂酵母细胞中的脂滴增加；阿托伐他汀和Et-CAF减少了脂滴。这项研究表明，Et-CAF的抗高血脂作用具有重要意义，可能用于填补对抗疗法药物造成的空白。
