核酸酶整合激酶超级组装体（NiKs）及其在RNA加工中的作用。
Nuclease integrated kinase super assemblies (NiKs) and their role in RNA processing.
作者信息
Pillon Monica C, Stanley Robin E
机构信息
Signal Transduction Laboratory, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Drive, Research Triangle Park, NC, 27709, USA.
出版信息
Curr Genet. 2018 Feb;64(1):183-190. doi: 10.1007/s00294-017-0749-9. Epub 2017 Sep 19.
Here we highlight the Grc3/Las1 complex, an essential RNA processing machine that is well conserved across eukaryotes and required for processing the pre-ribosomal RNA (pre-rRNA). Las1 is an endoribonuclease that cleaves the pre-rRNA while Grc3 is a polynucleotide kinase that phosphorylates the Las1-cleaved RNA product. Recently we showed that Grc3 and Las1 assemble into a higher-order complex composed of a dimer of Grc3/Las1 heterodimers that is required for nuclease and kinase activity. Unexpectedly, we found that the Grc3/Las1 complex draws numerous parallels with two other eukaryotic nucleases, Ire1 and RNase L. In this perspective we explore the similarities and differences between this family of nuclease integrated kinase super assemblies (NiKs) and their distinct roles in RNA cleavage.
在此，我们重点介绍Grc3/Las1复合物，这是一种重要的RNA加工机器，在真核生物中高度保守，是加工前核糖体RNA（pre-rRNA）所必需的。Las1是一种内切核糖核酸酶，可切割pre-rRNA，而Grc3是一种多核苷酸激酶，可将Las1切割后的RNA产物磷酸化。最近我们发现，Grc3和Las1组装成一种高阶复合物，该复合物由Grc3/Las1异二聚体的二聚体组成，是核酸酶和激酶活性所必需的。出乎意料的是，我们发现Grc3/Las1复合物与另外两种真核核酸酶Ire1和RNase L有许多相似之处。在此观点中，我们探讨了这个核酸酶整合激酶超级组装家族（NiKs）之间的异同及其在RNA切割中的不同作用。
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