Yousef G E, Bell E J, Mann G F, Murugesan V, Smith D G, McCartney R A, Mowbray J F
Department of Pathology, St Mary's Hospital Medical School, London.
Lancet. 1988 Jan 23;1(8578):146-50. doi: 10.1016/s0140-6736(88)92722-5.
76 patients with the postviral fatigue syndrome (PVFS) and 30 matched controls were investigated. Virus isolation was attempted from concentrated faecal samples by direct culture and after acid dissociation of virus from antibody. Positive cultures of enteroviruses were obtained from 17 (22%) patients and 2 (7%) controls. An enterovirus-group-specific monoclonal antibody, 5-D8/1, directed against the VP1 polypeptide, was used to detect enteroviral antigen in the circulation, either free or complexed with antibody. VP1 antigen was detected in the serum of 44 (51%) of a further group of 87 PVFS patients. The number of patients positive for VP1 antigen was greater (42/44) when IgM complexes were detectable than when they were not (2/23). 1 year later, the 17 patients of the first group of 76 with positive cultures were again studied. The same virus was again isolated from 5 (29%), 13 (76%) had detectable IgM responses to enteroviruses, and 9 (53%) were positive for VP1 antigen in the serum. These results show that chronic infection with enteroviruses occurs in many PVFS patients and that detection of enterovirus antigen in the serum is a sensitive and satisfactory method for investigating infection in these patients.
对76例病毒性疲劳综合征（PVFS）患者和30例匹配的对照者进行了研究。尝试通过直接培养以及从抗体中酸解离病毒后，从浓缩粪便样本中分离病毒。从17例（22%）患者和2例（7%）对照者中获得了肠道病毒阳性培养物。一种针对VP1多肽的肠道病毒组特异性单克隆抗体5-D8/1，用于检测循环中游离或与抗体结合的肠道病毒抗原。在另一组87例PVFS患者中，44例（51%）的血清中检测到VP1抗原。当可检测到IgM复合物时，VP1抗原阳性的患者数量更多（42/44），而当未检测到IgM复合物时则较少（2/23）。1年后，对第一组76例培养阳性的患者中的17例再次进行研究。5例（29%）再次分离出相同病毒，13例（76%）对肠道病毒有可检测到的IgM反应，9例（53%）血清中VP1抗原呈阳性。这些结果表明，许多PVFS患者存在肠道病毒慢性感染，并且血清中肠道病毒抗原的检测是研究这些患者感染情况的一种灵敏且令人满意的方法。
