Ito Takafumi, Kurita Jun, Haenen Olga L M
Tamaki Laboratory, Research Center for Fish Diseases, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, 224-1 Hiruda, Tamaki, Mie 519-0423, Japan.
Dis Aquat Organ. 2017 Sep 20;126(1):51-62. doi: 10.3354/dao03157.
Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis in goldfish Carassius auratus auratus. However, the virus has also been detected in Prussian carp C. gibelio and crucian carp C. carassius from European and Asian countries. To prevent spread of the causative virus to other areas, investigation of the risk factors of spread of this virus is important. In this study, 8 batches of goldfish imported into the Netherlands by airfreight from Asia and the Middle East were investigated for the presence of the virus. CyHV-2 DNA was detected by PCR in the pooled kidneys of 4 of the 8 imported goldfish batches, of which 1 was from a CyHV-2 disease case at a Dutch importer's quarantine facility. Sequence analysis of the CyHV-2 strains from this study and from previous reports showed that there were at least 6 different lengths in the mA region, resulting in tentatively at least 4 genotypes. Virus isolation was positive for only 1 (Amsterdam Schiphol-1 [AMS-1]) of the 8 samples. It was shown that the AMS-1 isolate was highly virulent to Ryukin goldfish after 100.3 TCID50 fish-1 intraperitoneal injection. The viral titre of the AMS-1 isolate for goldfish fin cells at several temperatures was similar to that of a Japanese CyHV-2 isolate. Our results prove that one of the routes of spread of various CyHV-2 strains is through the global trade of apparently healthy infected goldfish.
鲤疱疹病毒2型（CyHV-2）是金鱼（Carassius auratus auratus）疱疹病毒性造血坏死的病原体。然而，在来自欧洲和亚洲国家的德国镜鲤（C. gibelio）和鲫鱼（C. carassius）中也检测到了这种病毒。为防止致病病毒传播到其他地区，调查该病毒传播的风险因素很重要。在本研究中，对8批从亚洲和中东空运进口到荷兰的金鱼进行了病毒检测。通过PCR在8批进口金鱼中的4批的合并肾脏中检测到了CyHV-2 DNA，其中1批来自荷兰进口商检疫设施的一个CyHV-2病病例。对本研究及先前报告中的CyHV-2毒株进行序列分析表明，mA区域至少有6种不同长度，初步确定至少有4种基因型。8个样本中只有1个（阿姆斯特丹史基浦-1 [AMS-1]）病毒分离呈阳性。结果表明，经100.3 TCID50鱼-1腹腔注射后，AMS-1分离株对琉金金鱼具有高致病性。AMS-1分离株在几个温度下对金鱼鳍细胞的病毒滴度与日本CyHV-2分离株相似。我们的结果证明，各种CyHV-2毒株的传播途径之一是通过表面健康但已感染的金鱼的全球贸易。
