Laboratoire de Biotechnologie Végétale Appliquée à l'Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, Tunisia.
Centre de Biophysique Moléculaire (CBM), CNRS UPR 4301, 45071 Orleans, France.
Int J Mol Sci. 2020 Mar 3;21(5):1719. doi: 10.3390/ijms21051719.
In order to harness local resources to improve well-being and human health, we aim in this study to investigate if the microalgae sp. isolated from the Tunisian coastal zone possesses any anticancer activity. sp. was cultured under normal (DSC) or stressed (DSS) conditions and extracted using different procedures. The biological activity assessment was performed on the Triple Negative Breast Cancer (TNBC) using 4T1 murine cells as a model. Results indicate that: (i) aqueous extract was the most cytotoxic compared to ethanolic and hydroalcoholic extracts; (ii) DSS activity was superior to that of DSC. DSS extracts induced apoptosis rather than necrosis, as evidenced by DNA fragmentation, PARP-1 cleavage and caspase-3 activation. Evaluation in an orthotopic TNBC model validated the anticancer activity in vivo. Intratumoral injection of DSS extract resulted in reduced tumor growth and an enhanced immune system activation. On the transcriptional side, the expression level of the immunosuppressive enzyme was decreased, as well as those of and genes. These results suggest a potential anticancer activity of Tunisian sp. deserving further attention.
为了利用当地资源提高福祉和人类健康，我们旨在本研究中调查从突尼斯沿海地区分离出的微藻是否具有抗癌活性。 sp. 在正常（DSC）或应激（DSS）条件下培养，并使用不同的程序进行提取。使用 4T1 鼠细胞作为模型，对三阴性乳腺癌（TNBC）进行了生物活性评估。结果表明：（i）与乙醇和水醇提取物相比，水提物的细胞毒性最强；（ii）DSS 活性优于 DSC。DSS 提取物诱导细胞凋亡而不是坏死，这可通过 DNA 片段化、PARP-1 切割和 caspase-3 激活来证明。在原位 TNBC 模型中的评估验证了体内的抗癌活性。DSS 提取物的瘤内注射导致肿瘤生长减少和免疫系统激活增强。在转录水平上，抑制性酶 的表达水平降低， 和 基因的表达水平也降低。这些结果表明，突尼斯 sp. 具有潜在的抗癌活性，值得进一步关注。
