Hamdy Omnia, El-Azab Jala, Al-Saeed Tarek A, Hassan Mahmoud F, Solouma Nahed H
Department of Engineering Applications of Laser, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza Governorate 12613, Egypt.
Department of Biomedical Engineering, Faculty of Engineering, Helwan University, Cairo Governorate 11795, Egypt.
Materials (Basel). 2017 Sep 20;10(9):1104. doi: 10.3390/ma10091104.
Optical differentiation is a promising tool in biomedical diagnosis mainly because of its safety. The optical parameters' values of biological tissues differ according to the histopathology of the tissue and hence could be used for differentiation. The optical fluence rate distribution on tissue boundaries depends on the optical parameters. So, providing image displays of such distributions can provide a visual means of biomedical diagnosis. In this work, an experimental setup was implemented to measure the spatially-resolved steady state diffuse reflectance and transmittance of native and coagulated chicken liver and native and boiled breast chicken skin at 635 and 808 nm wavelengths laser irradiation. With the measured values, the optical parameters of the samples were calculated in vitro using a combination of modified Kubelka-Munk model and Bouguer-Beer-Lambert law. The estimated optical parameters values were substituted in the diffusion equation to simulate the fluence rate at the tissue surface using the finite element method. Results were verified with Monte-Carlo simulation. The results obtained showed that the diffuse reflectance curves and fluence rate distribution images can provide discrimination tools between different tissue types and hence can be used for biomedical diagnosis.
光学鉴别是生物医学诊断中一种很有前景的工具，主要是因为其安全性。生物组织的光学参数值会根据组织的组织病理学而有所不同，因此可用于鉴别。组织边界上的光通量率分布取决于光学参数。所以，提供此类分布的图像显示可以提供一种生物医学诊断的视觉手段。在这项工作中，搭建了一个实验装置，用于测量在635和808纳米波长激光照射下，天然和凝固的鸡肝以及天然和煮熟的鸡胸鸡皮的空间分辨稳态漫反射率和透射率。利用测量值，结合修正的库贝尔卡-蒙克模型和布格-比尔-朗伯定律，在体外计算样品的光学参数。将估计的光学参数值代入扩散方程，使用有限元方法模拟组织表面的光通量率。结果通过蒙特卡洛模拟进行了验证。所获得的结果表明，漫反射曲线和光通量率分布图像可以提供不同组织类型之间的鉴别工具，因此可用于生物医学诊断。
