National Center for Drug Research and Evaluation, Italian National Institute of Health, Rome 00161, Italy.
Italian Society of Biointegrated Medicine, Bagnoli del Trigno, Isernia 86091, Italy.
Molecules. 2017 Sep 20;22(9):1578. doi: 10.3390/molecules22091578.
The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) was about 40% ( < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116: 35.1%; SW480: 11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116: 86%; SW480: 96%). Normal cells were not altered by (PsT + NAC) treatments.
本研究旨在证明一种对正常细胞无毒的天然化合物对癌细胞具有细胞毒性和致敏作用，最终目标是将其与化疗药物联合使用，以降低总体剂量。Trigno 生态型（PsT）李属果实提取物与一种由氨基酸、维生素和矿物质盐混合物组成的营养激活复合物（NAC），已显示出体外抗癌活性。（PsT+NAC）的细胞毒性作用已在人癌细胞中进行了评估，最初筛选了结直肠、子宫颈和支气管肺泡细胞，随后重点关注结肠癌细胞 HCT116 和 SW480。与对照细胞相比，用（PsT10mg/mL+NAC）处理后 HCT116 和 SW480 的细胞活力降低约 40%（<0.05）。当用磷酸盐缓冲盐水（PBS）或生理盐水（PS）代替药物载体（NAC）时，细胞存活率降低无效。癌细胞线粒体膜电位的流式细胞术评估显示，去极化线粒体增加了 20%。细胞周期分析显示出现了 Sub G1（Gap 1 期）峰（HCT116：35.1%；SW480：11.6%），表明诱导了细胞凋亡死亡，这通过 Annexin V 检测得到了证实（HCT116：86%；SW480：96%）。正常细胞不受（PsT+NAC）处理的影响。
