miR-337-3p 通过调控 PTEN/AKT 轴促进骨关节炎软骨细胞增殖并抑制凋亡。
MiR-337-3p promotes chondrocytes proliferation and inhibits apoptosis by regulating PTEN/AKT axis in osteoarthritis.
机构信息
Department of orthopedics, The Third Affiliated Hospital of Suchow University, Changzhou; 213000, China, China.
Department of orthopedics, The Third Affiliated Hospital of Suchow University, Changzhou; 213000, China, China.
出版信息
Biomed Pharmacother. 2017 Nov;95:1194-1200. doi: 10.1016/j.biopha.2017.09.016. Epub 2017 Oct 6.
BACKGROUND
Osteoarthritis (OA) is a degenerative disease of articular cartilage and its main pathological feature is cartilage destruction, but its specific pathogenesis is still debatable. The aim of this study was to explore the role of miR-337-3p in OA pathogenesis.
METHODS
The expression of miR-337-3p and PTEN in osteoarthritic cartilage tissues was detected using quantitative real time PCR and western blot, respectively. The regulation of miR-337-3p on PTEN was examined by luciferase reporter gene assays. The manipulation of miR-337-3p and PTEN was mediated by siRNA interference technology. The cell viability was analyzed by MTT assays.
RESULTS
MiR-337-3p expression was significantly down-regulated in osteoarthritic cartilage tissues compared with normal cartilage tissues. Further studies confirmed that miR-337-3p overexpression evidently promoted the proliferation and inhibited the apoptosis of OA chondrocytes. PTEN expression was significantly up-regulated in osteoarthritic cartilage tissues and was negatively regulated by miR-337-3p in chondrocytes. PTEN silencing could improve the proliferation of OA chondrocytes and increased pAKT protein expression in OA chondrocytes.
CONCLUSION
MiR-337-3p regulated OA chondrocytes proliferation through PTEN/AKT axis and thus involved in OA.
背景
骨关节炎（OA）是一种关节软骨的退行性疾病，其主要的病理特征是软骨破坏，但具体的发病机制仍存在争议。本研究旨在探讨 miR-337-3p 在 OA 发病机制中的作用。
方法
采用实时定量 PCR 和 Western blot 分别检测 OA 软骨组织中 miR-337-3p 和 PTEN 的表达。通过荧光素酶报告基因实验检测 miR-337-3p 对 PTEN 的调控作用。通过 siRNA 干扰技术对 miR-337-3p 和 PTEN 进行操作。通过 MTT 分析检测细胞活力。
结果
与正常软骨组织相比，miR-337-3p 在 OA 软骨组织中的表达明显下调。进一步的研究证实，miR-337-3p 的过表达明显促进了 OA 软骨细胞的增殖，并抑制了其凋亡。PTEN 在 OA 软骨组织中的表达明显上调，并在软骨细胞中受 miR-337-3p 的负调控。PTEN 沉默可改善 OA 软骨细胞的增殖，并增加 OA 软骨细胞中 pAKT 蛋白的表达。
结论
miR-337-3p 通过 PTEN/AKT 轴调节 OA 软骨细胞的增殖，从而参与 OA 的发生。
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