VISMapper：用于基因治疗的病毒插入位点的超快速详尽绘图
VISMapper: ultra-fast exhaustive cartography of viral insertion sites for gene therapy.
作者信息
Juanes José M, Gallego Asunción, Tárraga Joaquín, Chaves Felipe J, Marín-Garcia Pablo, Medina Ignacio, Arnau Vicente, Dopazo Joaquín
机构信息
Departamento de Informática, Escuela Técnica Superior de Ingeniería (ETSE), Universidad de Valencia, 46100, Valencia, Burjassot, Spain.
Computational Genomics Department, Prince Felipe Research Center, 46012, Valencia, Spain.
出版信息
BMC Bioinformatics. 2017 Sep 20;18(1):421. doi: 10.1186/s12859-017-1837-z.
BACKGROUND
The possibility of integrating viral vectors to become a persistent part of the host genome makes them a crucial element of clinical gene therapy. However, viral integration has associated risks, such as the unintentional activation of oncogenes that can result in cancer. Therefore, the analysis of integration sites of retroviral vectors is a crucial step in developing safer vectors for therapeutic use.
RESULTS
Here we present VISMapper, a vector integration site analysis web server, to analyze next-generation sequencing data for retroviral vector integration sites. VISMapper can be found at: http://vismapper.babelomics.org .
CONCLUSIONS
Because it uses novel mapping algorithms VISMapper is remarkably faster than previous available programs. It also provides a useful graphical interface to analyze the integration sites found in the genomic context.
背景
病毒载体整合到宿主基因组并成为其持久组成部分的可能性，使其成为临床基因治疗的关键要素。然而，病毒整合存在相关风险，比如可能意外激活致癌基因从而导致癌症。因此，分析逆转录病毒载体的整合位点是开发更安全治疗用载体的关键步骤。
结果
在此我们展示了VISMapper，一个用于分析逆转录病毒载体整合位点的下一代测序数据的载体整合位点分析网络服务器。可通过以下网址访问VISMapper：http://vismapper.babelomics.org 。
结论
由于使用了新颖的映射算法，VISMapper比之前可用的程序速度明显更快。它还提供了一个有用的图形界面来分析在基因组背景下发现的整合位点。
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