恶性疟原虫的多态性11.1位点。

The polymorphic 11.1 locus of Plasmodium falciparum.

作者信息

Kahane B, Sibilli L, Scherf A, Jaureguiberry G, Langsley G, Ozaki L S, Guillotte M, Müller-Hill B, Pereira da Silva L, Mercereau-Puijalon O

机构信息

Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.

出版信息

Mol Biochem Parasitol. 1987 Nov;26(1-2):77-85. doi: 10.1016/0166-6851(87)90132-0.

DOI:10.1016/0166-6851(87)90132-0

PMID:2893257

Abstract

Clone pPF11.1 encodes a Plasmodium falciparum antigen expressed during the intraerythrocytic cycle and containing tandem repeats of a 9 amino acid unit. We report here an analysis of the genomic region specific for 11.1, which extends over 30 kb. It contains two blocks of repeats, spanning 13 kb and 9 kb. The restriction map suggests that the locus may result from a gene duplication. The 11.1 region is present in all P. falciparum strains examined so far. Southern analysis of 8 distinct isolates indicates that the locus is highly polymorphic. Thus the pPF11.1 repeats constitute a sensitive and discriminating probe to type P. falciparum strains.

摘要

克隆体pPF11.1编码一种在红细胞内期表达的恶性疟原虫抗原，该抗原含有一个9个氨基酸单位的串联重复序列。我们在此报告对11.1特异性基因组区域的分析，该区域延伸超过30 kb。它包含两个重复序列区域，分别跨越13 kb和9 kb。限制性图谱表明该基因座可能源于基因复制。到目前为止，在所有检测的恶性疟原虫菌株中均存在11.1区域。对8个不同分离株的Southern分析表明该基因座具有高度多态性。因此，pPF11.1重复序列构成了一种用于恶性疟原虫菌株分型的灵敏且具有鉴别力的探针。