Biotechnology Program, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkhunthian, Bangkok 10150, Thailand.
ECoWaste, The Joint Graduate School of Energy and Environment, King Mongkut's University of Technology Thonburi, Thungkru, Bangkok 10140, Thailand.
Biomed Res Int. 2017;2017:2782850. doi: 10.1155/2017/2782850. Epub 2017 Aug 28.
Various anaerobic starter seeds from different sources were investigated for their efficacies in treatment of different types of wastewater. Six combinations of starter seeds and wastewaters were selected out of 25 combination batch experiments and operated in semicontinuous reactors. It was noticed that the efficacies of various anaerobic starter seeds for biogas production from different types of wastewater in terms of reactor performance and stability were depended on wastewater characteristics and F/M ratio affecting microbial community and their microbial activities. However, exogenous starter seed can be used across different types of wastewater with or without acclimatization. Four reactors reached the targeted OLR of 2 kg COD/m·d with high performance and stability except for concentrated rubber wastewater (RBw), even using high active starter seeds of cassava starch (CSs) and palm oil (POs). The toxic compounds in RBw such as ammonia and sulfate might also adversely affect methanogenic activity in CSsRBw and POsRBw reactors. DGGE analysis showed that propionate utilizers, strain LYP and sp., were detected in all samples. For domain, methylotrophic, hydrogenotrophic, and acetoclastic methanogens were also detected. Syntrophic relationships were assumed between propionate utilizers and methanogens as acetate/H2 producers and utilizers, respectively.
从不同来源筛选了各种厌氧启动种子，以研究它们对不同类型废水的处理效果。在 25 组组合批实验中，选择了 6 种启动种子和废水的组合，并在半连续式反应器中进行操作。结果表明，不同类型厌氧启动种子对不同类型废水沼气生产的功效，取决于影响微生物群落及其微生物活性的废水特性和 F/M 比。然而，外源启动种子可以在无需驯化的情况下用于不同类型的废水。除了浓缩橡胶废水（RBw）外，其余四个反应器均达到了 2 kg COD/m·d 的目标 OLR，具有较高的性能和稳定性，即使使用了高活性的木薯淀粉（CSs）和棕榈油（POs）启动种子。RBw 中的有毒化合物，如氨和硫酸盐，可能也会对 CSsRBw 和 POsRBw 反应器中的产甲烷活性产生不利影响。DGGE 分析表明，所有样品中均检测到了丙酸利用菌、菌株 LYP 和 sp.。在所有样品中还检测到了甲基营养型、氢营养型和乙酰营养型产甲烷菌。假定丙酸利用菌与产甲烷菌之间存在协同关系，分别作为乙酸/H2 的产生菌和利用菌。
