Department of Environmental Engineering, Faculty of Engineering and Architecture, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, Turkey.
World J Microbiol Biotechnol. 2012 Feb;28(2):637-47. doi: 10.1007/s11274-011-0857-5. Epub 2011 Aug 21.
In this study, two laboratory-scale anaerobic batch reactors started up with different inoculum sludges and fed with the same synthetic wastewater were monitored in terms of performance and microbial community shift by denaturant gradient gel electrophoresis fingerprinting and subsequent cloning, sequencing analysis in order to reveal importance of initial quality of inoculum sludge for operation of anaerobic reactors. For this purpose, two different seed sludge were evaluated. In Reactor1 seeded with a sludge having less diverse microbial community (19 operational taxonomic unit (OTU's) for Bacterial and 8 OTU's for Archaeal community, respectively) and a methanogenic activity of 150 ml CH(4) g TVS(-1) day(-1), a chemical oxygen demand (COD) removal efficiency of 78.8 ± 4.17% was obtained at a substrate to microorganism (S/X) ratio of 0.38. On the other hand, Reactor2, seeded with a sludge having a much more diverse microbial community (24 OTU's for Bacterial and 9 OTU's for Archaeal communities, respectively) and a methanogenic activity, 450 ml CH(4) g TVS(-1) day(-1), operated in the same conditions showed a better start-up performance; a COD removal efficiency of over 98% at a S/X ratio of 0.53. Sequence analysis of Seed2 revealed the presence of diverse fermentative and syntrophic bacteria, whereas excised bands of Seed1 related to fermentative and sulfate/metal-reducing bacteria. This study revealed that a higher degree of bacterial diversity, especially the presence of syntrophic bacteria besides the abundance of key species such as methanogenic Archaea may play an important role in the performance of anaerobic reactors during the start-up period.
在这项研究中，使用两种不同的接种污泥启动了两个实验室规模的厌氧批式反应器，通过变性梯度凝胶电泳指纹图谱和随后的克隆、测序分析监测其性能和微生物群落变化，以揭示接种污泥初始质量对厌氧反应器运行的重要性。为此，评估了两种不同的种子污泥。在反应器 1 中，接种了微生物群落多样性较低的污泥（细菌的 19 个操作分类单元（OTU）和古菌的 8 个 OTU），甲烷生成活性为 150 ml CH 4 g TVS -1天-1，在底物与微生物（S/X）比为 0.38 时，COD 去除效率为 78.8±4.17%。另一方面，在反应器 2 中，接种了微生物群落多样性更高的污泥（细菌的 24 个 OTU 和古菌的 9 个 OTU），甲烷生成活性为 450 ml CH 4 g TVS -1天-1，在相同条件下运行时表现出更好的启动性能；在 S/X 比为 0.53 时，COD 去除效率超过 98%。对种子 2 的序列分析表明存在多种发酵和共生细菌，而种子 1 相关的发酵和硫酸盐/金属还原细菌的条带被切除。本研究表明，较高的细菌多样性程度，特别是除产甲烷古菌等关键物种的丰度外，共生细菌的存在可能在厌氧反应器的启动阶段对其性能发挥重要作用。
