Talbott Heather, Hou Xiaoying, Qiu Fang, Zhang Pan, Guda Chittibabu, Yu Fang, Cushman Robert A, Wood Jennifer R, Wang Cheng, Cupp Andrea S, Davis John S
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA.
Biochemistry and Molecular Biology Department, University of Nebraska Medical Center, 985870 Nebraska Medical Center, Omaha, NE 68198-5870, USA.
Data Brief. 2017 Sep 1;14:695-706. doi: 10.1016/j.dib.2017.08.026. eCollection 2017 Oct.
RNA expression analysis was performed on the corpus luteum tissue at five time points after prostaglandin F2 alpha treatment of midcycle cows using an Affymetrix Bovine Gene v1 Array. The normalized linear microarray data was uploaded to the NCBI GEO repository (GSE94069). Subsequent statistical analysis determined differentially expressed transcripts ± 1.5-fold change from saline control with ≤ 0.05. Gene ontology of differentially expressed transcripts was annotated by DAVID and Panther. Physiological characteristics of the study animals are presented in a figure. Bioinformatic analysis by Ingenuity Pathway Analysis was curated, compiled, and presented in tables. A dataset comparison with similar microarray analyses was performed and bioinformatics analysis by Ingenuity Pathway Analysis, DAVID, Panther, and String of differentially expressed genes from each dataset as well as the differentially expressed genes common to all three datasets were curated, compiled, and presented in tables. Finally, a table comparing four bioinformatics tools' predictions of functions associated with genes common to all three datasets is presented. These data have been further analyzed and interpreted in the companion article "Early transcriptome responses of the bovine mid-cycle corpus luteum to prostaglandin F2 alpha includes cytokine signaling" [1].
使用Affymetrix牛基因v1阵列，对前列腺素F2α处理的发情周期中期奶牛黄体组织在五个时间点进行RNA表达分析。将标准化的线性微阵列数据上传至NCBI GEO数据库（GSE94069）。随后的统计分析确定了与生理盐水对照相比差异表达的转录本，变化倍数为±1.5倍，且P值≤0.05。通过DAVID和Panther对差异表达转录本进行基因本体注释。研究动物的生理特征在图中展示。通过Ingenuity Pathway Analysis进行的生物信息学分析经过整理、汇编并呈现在表格中。对与类似微阵列分析的数据集进行比较，并通过Ingenuity Pathway Analysis、DAVID、Panther和String对每个数据集的差异表达基因以及所有三个数据集共有的差异表达基因进行生物信息学分析，结果经过整理、汇编并呈现在表格中。最后，给出了一个表格，比较了四种生物信息学工具对所有三个数据集共有的基因相关功能的预测。这些数据在配套文章《牛发情周期中期黄体对前列腺素F2α的早期转录组反应包括细胞因子信号传导》[1]中得到了进一步分析和解读。
