Université Paris Sud-CNRS, UMR 8000, Rue Henri Becquerel, Bâtiment 201 P 2, 91405, Orsay, France.
J Am Soc Mass Spectrom. 2017 Nov;28(11):2519-2522. doi: 10.1007/s13361-017-1810-0. Epub 2017 Sep 20.
Noncovalent interactions are essential for the structural organization of biomacromolecules in cells. For this reason, the study of the biophysical, dynamic, and architectural interactions among biomacromolecules is essential. Since mass spectrometry requires compatible solutions while preserving the noncovalent bonding network, we envisioned that size exclusion chromatography coupled with ion mobility and mass spectrometry would be a valuable technique to desalt the initial sample and provide solution and gas-phase structural information in a single stage experiment. Such coupling allowed obtaining information on solution protein complex composition with SEC separation and on authenticity and purity with IMS-MS. Our study demonstrated that such coupling is compatible, useful, as well as suitable for a routine analysis, in pharmaceutical industry, for example. Mobility data were reliable and injected standards allowed calibrating the collision cross-section scale. Graphical Abstract ᅟ.
非共价相互作用对于细胞中生物大分子的结构组织至关重要。出于这个原因，研究生物大分子之间的生物物理、动态和结构相互作用至关重要。由于质谱需要兼容的溶液，同时保持非共价键网络，我们设想，尺寸排阻色谱与离子淌度和质谱的联用将是一种有价值的技术，可以对初始样品进行脱盐，并在单个实验中提供溶液和气相结构信息。这种联用允许通过 SEC 分离获得有关溶液蛋白复合物组成的信息，以及通过 IMS-MS 获得有关真实性和纯度的信息。我们的研究表明，这种联用在制药行业等领域是兼容的、有用的，也适合常规分析。迁移率数据是可靠的，并且注入的标准允许对碰撞截面尺度进行校准。
