Yan Hai-Xia, Luo Hong-Mei, Li Ying, Sun Yong-Zhen, Sun Chao, Qian Zhong-Zhi, Chen Shi-Lin
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Beijing Institute for Drug Control, Beijing 102206, China.
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4158-4164. doi: 10.4268/cjcmm20162212.
Using the latest 454 GS FLX platform and Titanium regent, a substantial expressed sequence tag (ESTs) dataset of Ephedra sinica was produced, and the profile of gene expression and function gene of which were investigated. A total of 48 389 reads with an average length of 373 bp were generated. These 454 reads were assembled into 18 801 unigenes, which were all 454 sequencing identified. A total number of 10 531 unigenes(56.0%) were annotated using BLAST searches (E-value≤1×10⁻⁵) against the Nr, Nt, TAIR, SwissProt and KEGG databases. With respect to genes related to ephedrine biosynthesis, 19 unigenes(encoding 9 enzymes) were found. A total of 97 putative genes encoding cytochrome P450s were also discovered. Data presented in this study will provide an important resource for the scientific community that is interested in the functional genomics and secondary metabolism of E. sinica.
利用最新的454 GS FLX平台和钛试剂，构建了草麻黄的一个大量表达序列标签（ESTs）数据集，并对其基因表达谱和功能基因进行了研究。共产生了48389条平均长度为373 bp的 reads。这些454 reads被组装成18801个单基因，这些单基因均通过454测序鉴定。通过对Nr、Nt、TAIR、SwissProt和KEGG数据库进行BLAST搜索（E值≤1×10⁻⁵），共注释了10531个单基因（56.0%）。关于与麻黄碱生物合成相关的基因，发现了19个单基因（编码9种酶）。还发现了总共97个推定的细胞色素P450编码基因。本研究提供的数据将为对草麻黄功能基因组学和次生代谢感兴趣的科学界提供重要资源。
