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不同浓度氮磷对丹参生长及活性成分的影响

[Effects of different concentrations of nitrogen and phosphorus on growth and active components of Salvia miltiorrhiza].

作者信息

Xia Gui-Hui, Wang Qiu-Ling, Wang Wen-Quan, Hou Jun-Ling, Song Qing-Yan, Luo Lin, Zhang Dou-Dou, Yang Xiang

机构信息

School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4175-4182. doi: 10.4268/cjcmm20162215.

Abstract

With annual Salvia miltiorrhiza seedlings as experimental material, using "3414" optimal regression design recommended by the Ministry of Agriculture and regularly watered with nutrient solution, through the dynamic sampling of S. miltiorrhiza in different growing stages, and the growth index, dry weight of plant root and content of active components were measured. The potted experiments were applied to study the effects of different nitrogen and phosphorus ratios on the growth, dry matter accumulation and accumulation of active components of S. miltiorrhiza, in order to explore a compatible fertilization method of nitrogen and phosphorus ratio that are suitable for production and quality of S. miltiorrhiza. The results reported as follows:①High concentrations of nitrogen fertilizer was beneficial to dry matter accumulation of S. miltiorrhiza aerial parts, and low concentration of nitrogen fertilizer transferred the dry matter accumulation to underground, and N1P1 could make the transfer ahead of time;②Regression analysis showed that in the early growth stage (before early July), we could use the nitrogen and phosphorus as basic fertilizer at a concentration of 1.521,0.355 g•L⁻¹ respectively to promote the growth of S. miltiorrhiza and at a concentration of 2.281,0.710 g•L⁻¹ respectively to promote the dry matter accumulation of root (after mid-August);③Five kinds of active components of S. miltiorrhiza decreased with the increase of nitrogen concentration, and increased with the increase of the concentration of phosphate fertilizer. Nitrogenous fertilizer, phosphate fertilizer in N-P=2∶3 ratio was more suitable for the accumulation of salvianolic acids, in N-P=1∶2 ratio was more suitable for the accumulation of tanshinone.

摘要

以一年生丹参幼苗为试验材料,采用农业部推荐的“3414”最优回归设计,定期浇灌营养液,通过对丹参不同生育期动态取样,测定其生长指标、植株根干重及活性成分含量。采用盆栽试验研究不同氮磷配比对丹参生长、干物质积累及活性成分积累的影响,以探索适合丹参生产和品质的氮磷配比协调施肥方法。结果如下:①高浓度氮肥有利于丹参地上部干物质积累,低浓度氮肥使干物质积累向地下部转移,N1P1可使这种转移提前;②回归分析表明,在生长前期(7月初以前),分别以1.521、0.355 g•L⁻¹的氮磷浓度作基肥可促进丹参生长,在8月中旬以后分别以2.281、0.710 g•L⁻¹的氮磷浓度作基肥可促进根部干物质积累;③丹参的5种活性成分随氮浓度增加而降低,随磷肥浓度增加而升高。氮磷肥中N-P=2∶3比例更适合丹酚酸的积累,N-P=1∶2比例更适合丹参酮的积累。

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