Zhou Bing-Qian, Lv Hai-Hua, Yang Fan, Liu Wei, Geng Yan-Ling, Wang Xiao, Wang Tao, Qu Zhen-Yu
Shandong Analysis and Test Center, Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Ji'nan 250014, China.
Shandong Medicinal Materials Co., Ltd., Ji'nan 250012, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1883-1893. doi: 10.19540/j.cnki.cjcmm.2017.0079.
To study the effects of different variable temperature drying modes on active components of roots of Salvia miltiorrhiza f. alba, and provide basis for its industrialized drying process. In order to ensure the content of active components, variable temperature drying modes were designed: low temperature at 30 ℃ and high temperature at 60 ℃, low temperature at 30 ℃ and high temperature at 70 ℃, low temperature at 30 ℃ and high temperature at 80 ℃, low temperature at 40 ℃ and high temperature at 60 ℃, low temperature at 40 ℃ and high temperature at 70 ℃, low temperature at 40 ℃ and high temperature at 80 ℃ and air dry oven was used for variable temperature drying process. Then HPLC method was used to determine the changes of active components in roots of S. miltiorrhiza f. alba under different temperature modes; and SPSS 17.0 was used to analyze the data. The results showed that the samples, which were first dried at 40 ℃ for six hours and then dried at 80 ℃ for three hours, had the highest contents in dihydrotanshinone, cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA as compared with other kinds of drying methods, and the contents were 0.35, 2.76, 0.78, 4.47 mg•g⁻¹, respectively. Additionally, as compared with samples dried in the shade, the contents of dihydrotanshinone, cryptotanshinone and tanshinone Ⅰ were increased 2.9% (P>0.05), 45.3% (P<0.05) and 34.5% (P<0.05), respectively; however, the content of tanshinone ⅡA was decreased by 44.1% (P<0.05). The water-soluble active components (rosmarinic acid and salvianolic acid B) of roots of S. miltiorrhiza f. alba, had the highest contents when the samples were first dried at 30 ℃ for six hours and then 70 ℃ for three hours, and the contents were 3.83,55.44 mg•g⁻¹, increased by 62.3% (P<0.05) and 109.1% (P<0.05) respectively as compared with the samples dried in the shade. Variable temperature drying can significantly affect the contents of active components in roots of S. miltiorrhiza f. alba. As compared with the traditional process of shade-drying process, low temperature drying can significantly increase the content of water-soluble active components and also with significant promotion effect on the liposoluble components such as tanshinone ⅡA, cryptotanshinone and tanshinone Ⅰ. The variable temperature drying mode, can effectively shorten the process of drying and provide theoretical basis for industrial processing of roots of S. miltiorrhiza f. alba.
研究不同变温干燥方式对白花丹参根中活性成分的影响,为其工业化干燥工艺提供依据。为保证活性成分含量,设计变温干燥方式:30℃低温和60℃高温、30℃低温和70℃高温、30℃低温和80℃高温、40℃低温和60℃高温、40℃低温和70℃高温、40℃低温和80℃高温,并采用热风干燥箱进行变温干燥处理。然后用高效液相色谱法测定白花丹参根在不同温度模式下活性成分的变化;并用SPSS 17.0软件进行数据分析。结果表明,先在40℃干燥6小时,再在80℃干燥3小时的样品,其二氢丹参酮、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA的含量与其他干燥方法相比最高,含量分别为0.35、2.76、0.78、4.47mg•g⁻¹。此外,与阴干样品相比,二氢丹参酮、隐丹参酮和丹参酮Ⅰ的含量分别增加了2.9%(P>0.05)、45.3%(P<0.05)和34.5%(P<0.05);而丹参酮ⅡA的含量下降了44.1%(P<0.05)。白花丹参根的水溶性活性成分(迷迭香酸和丹酚酸B),在样品先在30℃干燥6小时,然后在70℃干燥3小时时含量最高,含量分别为3.83、55.44mg•g⁻¹,与阴干样品相比分别增加了62.3%(P<0.05)和109.1%(P<0.05)。变温干燥能显著影响白花丹参根中活性成分的含量。与传统的阴干工艺相比,低温干燥能显著提高水溶性活性成分的含量,对丹参酮ⅡA、隐丹参酮和丹参酮Ⅰ等脂溶性成分也有显著的促进作用。变温干燥方式能有效缩短干燥过程,为白花丹参根的工业化加工提供理论依据。