Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Int J Mol Sci. 2018 Jan 16;19(1):270. doi: 10.3390/ijms19010270.
Plant growth-promoting fungi (PGPF) have attracted considerable interest as bio-fertilisers due to their multiple beneficial effects on plant quantity and quality and their positive relationship with the ecological environment. Advancements in the development of PGPF for crops and economic plant cultivation applications have been achieved, but such improvements for the use of PGPF with popular medicinal herbs, such as , are rare. In this study, we collected specimens inhabiting wild, semi-wild, farmland and pot-cultured areas in the Henan province of China and isolated endophytes from the roots, shoots and leaves of these samples. Twenty-eight strains of the dominant genus were identified and selected as candidate PGPF. Under greenhouse conditions, sp. A13 simultaneously enhanced the dry root biomass and secondary metabolite accumulation of as the optimal PGPF of the 28 candidate isolates. To further assess the interaction between and sp. A13, the effects on seedlings growth, active ingredient accumulation, and the activity of key enzymes for effective biosynthetic pathways were investigated over a period of six months under field conditions. Compared to uninoculated seedlings, seedlings colonised by sp. A13 showed significant increment of 140% in fresh weight, 138% in dry weight, and enhancement in the contents of total phenolic acid, lithospermic acids A and B (LAA and LAB, respectively) of 210%, 128% and 213%, respectively. Examination of the related enzyme activities showed that the elicitation effect of A13 on LAB accumulation correlated with cinnamic acid 4-hydroxylase (C4H) activity in the phenylpropanoid pathway under field conditions. Our results confirmed that sp. A13 not only contributes to the stimulation of root growth, but also boosts the secondary metabolism, thus demonstrating its application potential as a bio-fertiliser for cultivation, especially in areas outside of its native growth regions.
植物促生真菌（PGPF）因其对植物数量和质量的多种有益影响以及与生态环境的积极关系，作为生物肥料引起了相当大的关注。在作物和经济植物栽培应用中，PGPF 的开发取得了进展，但在常用药用植物如 的应用中，这种改进很少见。在这项研究中，我们收集了中国河南省野生、半野生、农田和盆栽地区的 标本，并从这些样本的根、茎和叶中分离出内生菌。鉴定出 28 株优势属 菌株，并选择它们作为候选 PGPF。在温室条件下， sp. A13 同时增强了 的干根生物量和次生代谢产物积累，是 28 个候选分离株中最佳的 PGPF。为了进一步评估 和 sp. A13 之间的相互作用，在田间条件下，经过六个月的时间，研究了它们对幼苗生长、活性成分积累以及有效生物合成途径关键酶活性的影响。与未接种的幼苗相比， 幼苗被 sp. A13 定殖后，鲜重增加了 140%，干重增加了 138%，总酚酸、丹参素 A 和 B（LAA 和 LAB，分别）的含量分别提高了 210%、128%和 213%。相关酶活性的检测表明，A13 对 LAB 积累的诱导作用与田间条件下苯丙烷途径中肉桂酸 4-羟化酶（C4H）活性相关。我们的结果证实， sp. A13 不仅有助于刺激 根的生长，而且还能促进次生代谢，因此证明了其作为 生物肥料的应用潜力，特别是在其原生生长地区以外的地区。
