Emrie P A, Jones C, Hofmann T, Fisher J H
University of Colorado Health Sciences Center, Department of Medicine, Denver 80206.
Somat Cell Mol Genet. 1988 Jan;14(1):105-10. doi: 10.1007/BF01535054.
The 18-kd hydrophobic pulmonary surfactant protein (PSP-B) is a developmentally regulated protein which is important for normal lung function. A complementary DNA probe for 221 NH2 terminal amino acids of PSP-B was used to determine the chromosomal location of this gene and identify a restriction fragment length polymorphism (RFLP). Southern blot hybridization to genomic DNA isolated from a panel of human-CHO somatic cell hybrids unambiguously maps this gene to chromosome 2. Human DNA cut with BamHI yields a RFLP with variable bands at 2.8 and 2.6 kb. Since there is a relative lack of polymorphic markers for chromosome 2, this sequence may be useful in linkage analysis.
18-kd疏水肺表面活性蛋白（PSP-B）是一种受发育调控的蛋白，对正常肺功能很重要。用针对PSP-B 221个氨基末端氨基酸的互补DNA探针来确定该基因的染色体定位，并鉴定一种限制性片段长度多态性（RFLP）。与从一组人-中国仓鼠卵巢（CHO）体细胞杂种中分离出的基因组DNA进行Southern印迹杂交，明确将该基因定位到2号染色体。用BamHI切割的人DNA产生一个RFLP，在2.8 kb和2.6 kb处有可变条带。由于2号染色体相对缺乏多态性标记，该序列可能在连锁分析中有用。
