Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Biosens Bioelectron. 2018 Feb 15;100:251-258. doi: 10.1016/j.bios.2017.09.009. Epub 2017 Sep 9.
By means of molecular imprinting of a conducting polymer, molecular cavities selective for oxytocin nonapeptide, an autism biomarker, were designed. Embedding of the oxytocin template, and then its extracting from the molecularly imprinted polymer (MIP) was confirmed by the XPS analysis. AFM imaging of the MIP film surface indicated changes in mechanical properties of the film after template extraction. The MIP synthetic receptor was deposited by potentiodynamic electropolymerization as a thin film on an Au film electrode in an electrochemical miniaturized microfluidic cell. The use of this cell allowed to shorten analysis time and to decrease the sample volume. The linear dynamic concentration range extended from 0.06 to 1mM with the limit of detection of 60µM (S/N = 3). Advantageously, sensitivity of the diagnostic microfluidic platform devised for oxytocin determination in both synthetic serum samples and in aqueous solutions was similar and, moreover, it was selective to common interferences, such as oxytocin analogs and potential metabolites.
通过对导电聚合物进行分子印迹，设计了对催产素九肽（一种自闭症生物标志物）具有选择性的分子腔。通过 XPS 分析证实了模板的嵌入，然后从分子印迹聚合物（MIP）中提取。MIP 薄膜表面的 AFM 成像表明，模板提取后薄膜的机械性能发生了变化。MIP 合成受体通过恒电位电聚合沉积在 Au 薄膜电极上，形成电化学微型化微流控池中的薄膜。使用这种池可以缩短分析时间并减少样品体积。线性动态浓度范围从 0.06 到 1mM，检测限为 60µM（S/N = 3）。有利的是，为在合成血清样品和水溶液中测定催产素而设计的诊断微流控平台的灵敏度相似，并且对常见的干扰物（如催产素类似物和潜在代谢物）具有选择性。
