Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan; Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Fish Shellfish Immunol. 2017 Nov;70:638-647. doi: 10.1016/j.fsi.2017.09.045. Epub 2017 Sep 18.
A viral responsive protein (MjVRP) was characterized from Marsupenaeus japonicus haemocytes. In amino acid homology and phylogenetic tree analyses, MjVRP clustered in the same group with the viral responsive protein of Penaeus monodon (PmVRP15), showing 34% identity. MjVRP transcripts were mainly expressed in haemocytes and the lymphoid organ. Western blotting likewise showed that MjVRP was strongly expressed in haemocytes and the lymphoid organ. Immunostaining detected MjVRP within the cytosol next to the perinuclear region in some haemocytes. Experimental challenge with white spot syndrome virus (WSSV) significantly up-regulated the mRNA level of MjVRP in the M. japonicus haemocytes at 6 and 48 h. Flow cytometry and indirect immunofluorescence assays revealed that the ratio of MjVRP haemocytes significantly increased 24 and 48 h post-WSSV infection. These results suggest that MjVRP haemocytes have a supporting role in the pathogenesis of WSSV.
从日本对虾血细胞中鉴定出一种病毒反应蛋白（MjVRP）。在氨基酸同源性和系统发育树分析中，MjVRP 与斑节对虾的病毒反应蛋白（PmVRP15）聚在同一组，显示出 34%的同源性。MjVRP 转录本主要在血细胞和淋巴器官中表达。Western blot 同样表明 MjVRP 在血细胞和淋巴器官中强烈表达。免疫染色在一些血细胞的核周区附近的细胞质中检测到 MjVRP。用白斑综合征病毒（WSSV）进行实验性攻毒后，日本对虾血细胞中 MjVRP 的 mRNA 水平在 6 和 48 h 时显著上调。流式细胞术和间接免疫荧光检测显示，WSSV 感染后 24 和 48 h，MjVRP 血细胞的比例显著增加。这些结果表明，MjVRP 血细胞在 WSSV 发病机制中具有支持作用。
