Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan; Key Laboratory of Exploproportionn and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Fish Shellfish Immunol. 2018 Jul;78:233-237. doi: 10.1016/j.fsi.2018.04.045. Epub 2018 Apr 22.
Penaeidins are a unique family of antimicrobial peptides specific to penaeid shrimp and have been reported mainly function as anti-bacterial and anti-fungal. In order to investigate whether penaeidins could also respond to virus or not, we examined the effect of WSSV on MjPen-II (penaeidin in kuruma shrimp, Marsupenaeus japonicus) expression. In the control group, MjPen-II transcript level can be detected in almost all test tissues but was expressed most strongly in hemocytes. After WSSV infection, MjPen-II transcript level was significantly downregulated in hemocytes. Moreover, the proportion of MjPen-II+ hemocytes was not significantly different between non-infected and WSSV-infected shrimp, but the number of MjPen-II+ highly expressing hemocytes decreased after infection. In addition, MjPen-II was observed in the cytoplasm of granule-containing hemocytes. These results suggest that WSSV suppresses MjPen-II expression in hemocytes.
抗菌肽是对虾特有的一类独特的抗菌肽家族，主要具有抗细菌和抗真菌作用。为了研究抗菌肽是否也能对抗病毒，我们研究了 WSSV 对 MjPen-II（日本对虾抗菌肽）表达的影响。在对照组中，几乎可以在所有检测组织中检测到 MjPen-II 转录本水平，但在血细胞中表达最强。WSSV 感染后，血细胞中 MjPen-II 的转录本水平显著下调。此外，未感染和感染 WSSV 的虾之间 MjPen-II+血细胞的比例没有显著差异，但感染后 MjPen-II+高表达血细胞的数量减少。此外，在含颗粒的血细胞的细胞质中观察到 MjPen-II。这些结果表明，WSSV 抑制了血细胞中 MjPen-II 的表达。
