Department of Kinesiology, Kansas State University , Manhattan, Kansas.
Department of Anatomy and Physiology, Kansas State University , Manhattan, Kansas.
J Appl Physiol (1985). 2017 Dec 1;123(6):1571-1578. doi: 10.1152/japplphysiol.00207.2017. Epub 2017 Sep 21.
Craig JC, Broxterman RM, Wilcox SL, Chen C, Barstow TJ. Effect of adipose tissue thickness, muscle site, and sex on near-infrared spectroscopy derived total-[hemoglobin + myoglobin]. J Appl Physiol 123: 1571-1578, 2017. First published September 21, 2017; doi: 10.1152/japplphysiol.00207.2017 .-Adipose tissue thickness (ATT) attenuates signals from near-infrared spectroscopy (NIRS) and diminishes the absolute quantification of underlying tissues by contemporary NIRS devices. Based on the relationship between NIRS-derived total-[hemoglobin + myoglobin] (total-[Hb + Mb]) and ATT, we tested the hypotheses that the correction factor for ATT 1) is muscle site specific; 2) does not differ between men and women; and that 3) exclusion of the shortest source-detector distance from data analysis increases total-[Hb + Mb]. Fourteen healthy subjects (7 men) rested in a neutral body position (supine or prone) while measurements of total-[Hb + Mb] and ATT were taken at four muscles common to resting and exercise studies: vastus lateralis (VL), rectus femoris (RF), gastrocnemius (GS), and flexor digitorum superficialis (FDS). ATT averaged 6.0 ± 0.4 mm across all muscles. Every muscle showed a negative slope ( r: 0.6-0.94; P < 0.01) for total-[Hb + Mb] as a function of ATT: VL (-34 μM/mm), RF (-26 μM/mm), GS (-54 μM/mm), and FDS (-33 μM/mm). The projected total-[Hb + Mb] at 0 mm ATT ( y-intercept) was 452, 372, 620, and 456 μM for VL, RF, GS, and FDS, respectively. No differences were found between the sexes within VL, RF, or FDS, but men had a greater projected total-[Hb + Mb] at 0 mm for GS (688 ± 44 vs. 552 ± 40 μM; P < 0.05). Exclusion of the shortest source-detector distance increased total-[Hb + Mb] by 12 ± 1 μM ( P < 0.05). The present findings demonstrate that total-[Hb + Mb] should be corrected for ATT using muscle site-specific factors which are not sex specific, except in the case of GS. NEW & NOTEWORTHY Near-infrared spectroscopy (NIRS) is an important tool for physiologists and clinicians. However, adipose tissue greatly attenuates the signals from these devices. Correcting for this attenuation has been suggested based on the strength of the relationship between NIRS-derived measurements and the adipose tissue thickness. We show that this relationship is unique to the muscle site of interest but may not be sex specific. Accurate quantification of underlying tissue mandates researchers correct for adipose tissue thickness.
克雷格 JC，布罗克斯泰尔 RM，威尔科克斯 SL，陈 C，巴斯托 TJ。脂肪组织厚度、肌肉部位和性别对近红外光谱衍生的总血红蛋白+肌红蛋白的影响。应用生理学杂志 123:1571-1578,2017。首次出版于 2017 年 9 月 21 日；doi:10.1152/japplphysiol.00207.2017。-脂肪组织厚度（ATT）会衰减近红外光谱（NIRS）的信号，并降低当代 NIRS 设备对底层组织的绝对定量。基于 NIRS 衍生的总血红蛋白+肌红蛋白（总[Hb+Mb]）与 ATT 之间的关系，我们检验了以下假设：1）校正因子是肌肉部位特异性的；2）男性和女性之间没有差异；3）排除数据分析中最短的源-探测器距离可以增加总[Hb+Mb]。14 名健康受试者（7 名男性）在中立体位（仰卧或俯卧）休息时，在四个常用于休息和运动研究的肌肉部位测量总[Hb+Mb]和 ATT：股外侧肌（VL）、股直肌（RF）、腓肠肌（GS）和指浅屈肌（FDS）。所有肌肉的 ATT 平均为 6.0±0.4mm。每个肌肉都显示出总[Hb+Mb]与 ATT 的负斜率（r：0.6-0.94；P<0.01）：VL（-34μM/mm）、RF（-26μM/mm）、GS（-54μM/mm）和 FDS（-33μM/mm）。VL、RF 或 FDS 中，预测的 0mm ATT（y 截距）分别为 452、372、620 和 456μM。VL、RF 或 FDS 中，男女之间没有差异，但 GS 中男性预测的 0mm ATT 的总[Hb+Mb]更高（688±44 vs.552±40μM；P<0.05）。排除最短的源-探测器距离增加了 12±1μM（P<0.05）的总[Hb+Mb]。本研究结果表明，总[Hb+Mb]应使用肌肉部位特异性的校正因子进行校正，这些校正因子不是性别特异性的，GS 除外。新的和值得注意的-近红外光谱（NIRS）是生理学家和临床医生的重要工具。然而，脂肪组织大大衰减了这些设备的信号。根据 NIRS 衍生测量值与脂肪组织厚度之间的关系，已经提出了这种衰减的校正方法。我们表明，这种关系是特定于感兴趣的肌肉部位的，但可能不是性别特异性的。为了准确量化潜在组织，研究人员必须校正脂肪组织厚度。
