Human Exercise Physiology Research Laboratory, Department of Kinesiology, 1A Natatorium, Kansas State University, Manhattan, KS 66506-0302, USA.
Respir Physiol Neurobiol. 2013 Apr 1;186(2):180-7. doi: 10.1016/j.resp.2013.01.012. Epub 2013 Jan 26.
We calculated the light absorbing potential (LAP) of hemoglobin (Hb) and myoglobin (Mb) in mammalian skeletal muscle at rest based on analysis of published chemical and morphometric data (Part 1), interpreted changes in total[Hb+Mb] from NIRS during exercise (Part 2), and estimated the potential contribution of Hb and Mb to changes in NIRS from rest to exercise (Part 3). Part 1: [Hb] in skeletal muscle was estimated from microvascular volume, systemic blood [Hb], and microvascular hematocrit and saturation at rest and during exercise. Part 2: Changes in total[Hb+Mb] (as t[Hb+Mb]) during cycling or knee extension exercise were interpreted using the results of Part 1. Part 3: Using estimates of mean microvascular PO2, Hb and Mb contribution at peak exercise was estimated. Across several species, [Mb] contributed ∼50-70% of the total LAP to NIRS at rest in skeletal muscle. With exercise, increases in t[Hb+Mb] of up to 30% could be entirely explained by the predicted increase in microvascular hematocrit with exercise. Finally, Mb was estimated to contribute ∼70% of the changes in NIRS from rest to peak exercise.
我们根据已发表的化学和形态计量数据，计算了哺乳动物骨骼肌在休息时血红蛋白（Hb）和肌红蛋白（Mb）的光吸收潜力（LAP）（第 1 部分），解释了 NIRS 在运动期间总[Hb+Mb]的变化（第 2 部分），并估计了 Hb 和 Mb 对从休息到运动的 NIRS 变化的潜在贡献（第 3 部分）。第 1 部分：骨骼肌中的[Hb]根据微血管体积、全身血液[Hb]以及微血管血细胞比容和休息时及运动时的饱和度来估计。第 2 部分：使用第 1 部分的结果解释了在骑自行车或膝关节伸展运动期间总[Hb+Mb]（t[Hb+Mb]）的变化。第 3 部分：使用平均微血管 PO2 的估计值，估计了 Mb 在峰值运动时的贡献。在几个物种中，Mb 在休息时对 NIRS 的总 LAP 的贡献约为 50-70%。随着运动，t[Hb+Mb]的增加高达 30%，可以完全用运动时微血管血细胞比容的预测增加来解释。最后，Mb 被估计为从休息到峰值运动的 NIRS 变化贡献了约 70%。
