Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA.
Center for the Neural Basis of Cognition, Pittsburgh, PA, 15213, USA.
Nat Commun. 2017 Sep 21;8(1):652. doi: 10.1038/s41467-017-00765-4.
Cholinergic neurons in the basal forebrain project heavily to the main olfactory bulb, the first processing station in the olfactory pathway. The projections innervate multiple layers of the main olfactory bulb and strongly influence odor discrimination, detection, and learning. The precise underlying circuitry of this cholinergic input to the main olfactory bulb remains unclear, however. Here, we identify a specific basal forebrain cholinergic projection that innervates select neurons concentrated in the internal plexiform layer of the main olfactory bulb. Optogenetic activation of this projection elicits monosynaptic nicotinic and GABAergic currents in glomerular layer-projecting interneurons. Additionally, we show that the projection co-expresses markers for GABAergic neurotransmission. The data thus implicate neurotransmitter co-transmission in the basal forebrain regulation of this inhibitory olfactory microcircuit.Cholinergic neurons innervate multiple layers in the main olfactory bulb but the precise circuitry of this input is not known. Here the authors show that VGLUT3 cholinergic neurons selectively innervate deep short axon cells in specific layers and elicit robust monosynaptic GABAergic and nicotinic postsynaptic currents.
基底前脑的胆碱能神经元大量投射到主嗅觉球，这是嗅觉通路的第一个处理站。这些投射支配主嗅觉球的多个层，并强烈影响气味辨别、检测和学习。然而，这种胆碱能输入到主嗅觉球的精确潜在回路仍不清楚。在这里，我们确定了一个特定的基底前脑胆碱能投射，它支配集中在主嗅觉球内部神经丛状层的选择神经元。对这个投射的光遗传学激活在投射到肾小球层的中间神经元中引发单突触烟碱和 GABA 能电流。此外，我们还表明，该投射共表达 GABA 能神经递质传递的标志物。因此，这些数据表明基底前脑通过神经递质共传递来调节这个抑制性嗅觉微回路。胆碱能神经元支配主嗅觉球的多个层，但这个输入的精确回路尚不清楚。在这里，作者表明 VGLUT3 胆碱能神经元选择性地支配特定层中的深短轴细胞，并引发强烈的单突触 GABA 能和烟碱能突触后电流。
