Twell David, Brownfield Lynette
Department of Genetics, University of Leicester, Leicester, UK.
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Methods Mol Biol. 2017;1669:67-75. doi: 10.1007/978-1-4939-7286-9_6.
The male germline of flowering plants develops within the vegetative cell of the male gametophyte (pollen). The germline is established by asymmetric division of the microspore to form the generative cell. Mitotic division of the generative cell then produces the two sperm cells required for double fertilization. These differentiate to produce the proteins required for gamete attachment and fusion. An important aspect of understanding germline development is the characterization of germline gene expression. Here, we describe the use of a fluorescent reporter to study germline gene expression in developing pollen to assess the timing and specificity of expression.
开花植物的雄性生殖系在雄配子体（花粉）的营养细胞内发育。生殖系通过小孢子的不对称分裂形成生殖细胞而建立。生殖细胞的有丝分裂随后产生双受精所需的两个精子细胞。这些细胞分化产生配子附着和融合所需的蛋白质。理解生殖系发育的一个重要方面是生殖系基因表达的特征描述。在这里，我们描述了使用荧光报告基因来研究发育中的花粉中的生殖系基因表达，以评估表达的时间和特异性。
