Suzuki H
Gastroenterol Jpn. 1981;16(2):122-8. doi: 10.1007/BF02774385.
Serum gamma-glutamyl transpeptidase zymograms were examined by 4--26% polyacrylamide gradient gel slab electrophoresis using sera from 90 patients with various hepatobiliary diseases. The isozyme bands were separated into 13 bands in order from post albumin fraction. Characteristic patterns of the zymograms were obtained in cases of hepatocellular carcinoma and cholestasis. Bands I, II and II' may be specifically related to hepatocellular carcinoma. Band I was observed frequently in hepatocellular carcinoma, however it was also occasionally observed in alcoholic liver injury and metastatic liver cancer. Both band II and II' were only observed in hepatocellular carcinoma cases. The origin of band II and/or II' was not clarified but band I might appear through some proteolytic process in hepatoma tissue and also liver affected by alcohol. In cholestatic cases the activity of bands III and IX were prominent. The frequency of band VI decreased according to the severity of jaundice and a negative correlation appeared to exist between bands III and VI. This phenomenon may be partially related to lipid metabolism disorder which is frequently observed in cholestasis. Results of analysis of the frequency of appearance of these bands did not permit differentiation between intra- and extrahepatic cholestasis.
采用4%-26%聚丙烯酰胺梯度凝胶平板电泳法，对90例各种肝胆疾病患者的血清进行检测，观察血清γ-谷氨酰转肽酶同工酶谱。同工酶带按从白蛋白后区带开始依次分为13条带。肝细胞癌和胆汁淤积患者的同工酶谱呈现出特征性模式。I、II和II'带可能与肝细胞癌有特异性关系。I带在肝细胞癌中经常出现，但在酒精性肝损伤和转移性肝癌中也偶尔可见。II和II'带仅在肝细胞癌病例中出现。II和/或II'带的来源尚不清楚，但I带可能是通过肝癌组织以及受酒精影响的肝脏中的某些蛋白水解过程出现的。在胆汁淤积病例中，III和IX带的活性明显增强。VI带的出现频率随黄疸严重程度降低，且III和VI带之间似乎存在负相关。这种现象可能部分与胆汁淤积中常见的脂质代谢紊乱有关。这些带出现频率的分析结果无法区分肝内和肝外胆汁淤积。
