Institute of Synthetic Biology, Biomedical Center, Guangdong Province Key Laboratory for Aquatic Economic Animals and South China Sea Bio-Resource Exploitation, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Mar Drugs. 2017 Sep 22;15(10):296. doi: 10.3390/md15100296.
Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. A higher ratio of astaxanthin to the total carotenoids is required for efficient astaxanthin production. β-Carotene ketolase and hydroxylase play important roles in astaxanthin production. We first compared the conversion efficiency to astaxanthin in several β-carotene ketolases from sp. SD212, sp. DC18, sp. PC1, sp. N81106 and with the recombinant cells that synthesize zeaxanthin due to the presence of the The sp. SD212 and genes are the best combination for astaxanthin production. After balancing the activities of β-carotene ketolase and hydroxylase, an ASTA-1 that carries neither a plasmid nor an antibiotic marker was constructed to produce astaxanthin as the predominant carotenoid (96.6%) with a specific content of 7.4 ± 0.3 mg/g DCW without an addition of inducer.
虾青素是一种具有重要商业价值的类胡萝卜素，因其具有优越的抗氧化潜力，广泛应用于水产养殖、食品、化妆品和制药行业。虾青素生产需要更高的虾青素与总类胡萝卜素的比例。β-胡萝卜素酮化酶和羟化酶在虾青素生产中起着重要作用。我们首先比较了来自 sp. SD212、 sp. DC18、 sp. PC1、 sp. N81106 和 的几种 β-胡萝卜素酮化酶转化为虾青素的效率，这些酶由于存在 基因而能够合成玉米黄质。 sp. SD212 和 基因是虾青素生产的最佳组合。在平衡β-胡萝卜素酮化酶和羟化酶的活性后，构建了一个既不携带质粒也不携带抗生素标记的 ASTA-1，以生产虾青素作为主要类胡萝卜素（96.6%），特定含量为 7.4±0.3mg/g DCW，无需添加诱导剂。
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