Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan.
Graduate School of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan.
Nat Chem. 2017 Oct;9(10):956-960. doi: 10.1038/nchem.2808. Epub 2017 Jul 3.
The double-helix structure of DNA, in which complementary strands reversibly hybridize to each other, not only explains how genetic information is stored and replicated, but also has proved very attractive for the development of nanomaterials. The discovery of metal-mediated base pairs has prompted the generation of short metal-DNA hybrid duplexes by a bottom-up approach. Here we describe a metallo-DNA nanowire-whose structure was solved by high-resolution X-ray crystallography-that consists of dodecamer duplexes held together by four different metal-mediated base pairs (the previously observed C-Ag-C, as well as G-Ag-G, G-Ag-C and T-Ag-T) and linked to each other through G overhangs involved in interduplex G-Ag-G. The resulting hybrid nanowires are 2 nm wide with a length of the order of micrometres to millimetres, and hold the silver ions in uninterrupted one-dimensional arrays along the DNA helical axis. The hybrid nanowires are further assembled into three-dimensional lattices by interactions between adenine residues, fully bulged out of the double helix.
DNA 的双螺旋结构中，互补链可以相互可逆杂交，不仅解释了遗传信息是如何存储和复制的，而且还非常吸引人为开发纳米材料。金属介导碱基对的发现促使人们通过自下而上的方法生成短的金属-DNA 杂合双链。在这里，我们描述了一种金属-DNA 纳米线，其结构通过高分辨率 X 射线晶体学得到解决，该纳米线由十二聚体双链组成，由四个不同的金属介导碱基对（之前观察到的 C-Ag-C 以及 G-Ag-G、G-Ag-C 和 T-Ag-T）固定在一起，并通过参与双链 G-Ag-G 的 G 突出部分彼此连接。得到的杂合纳米线宽 2nm，长度为几微米到几毫米，银离子沿 DNA 螺旋轴排列在无中断的一维阵列中。杂合纳米线通过腺嘌呤残基之间的相互作用进一步组装成三维晶格，这些腺嘌呤残基完全从双螺旋中突出。
