Department of Materials & Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, 221-8686, Kanagawa, Japan.
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, 102-8554, Tokyo, Japan.
Angew Chem Int Ed Engl. 2022 Aug 1;61(31):e202204798. doi: 10.1002/anie.202204798. Epub 2022 Jun 21.
DNA/RNA duplexes containing metal-ion-mediated base pairs (metallo-base pairs) have potential applications in developing nucleic acid-based nanodevices and genetic code expansion. Many metallo-base pairs are formed within duplexes stabilized by Watson-Crick base pairs. Recently, the crystal structure of an Ag -DNA nanowire with an uninterrupted one-dimensional silver array was determined. Here, we present a new DNA helical wire, the "Ag -DNA rod", containing an uninterrupted array of 11 Ag ions. The Ag -DNA rod consisted of only C-Ag -C, G-Ag -G, G-Ag -5-bromouracil ( U), and U-Ag - U metallo base pairs, with no Watson-Crick pairs. The Ag -DNA rods were connected by non-canonical G-G pairs in crystals. Notably, data from our absorbance, circular dichroism, nuclear magnetic resonance, and mass spectrometry analyses suggested that the Ag -DNA rods formed in solution, as well as within crystals.
含有金属离子介导碱基对（金属碱基对）的 DNA/RNA 双链体在开发基于核酸的纳米器件和遗传密码扩展方面具有潜在的应用。许多金属碱基对是由 Watson-Crick 碱基对稳定的双链体形成的。最近，确定了具有不间断一维银阵列的 Ag-DNA 纳米线的晶体结构。在这里，我们提出了一种新的 DNA 螺旋线，即“Ag-DNA 棒”，其中包含不间断的 11 个 Ag 离子阵列。Ag-DNA 棒仅由 C-Ag-C、G-Ag-G、G-Ag-5-溴尿嘧啶（U）和 U-Ag-U 金属碱基对组成，没有 Watson-Crick 碱基对。Ag-DNA 棒在晶体中通过非规范的 G-G 对连接。值得注意的是，我们的吸收、圆二色性、核磁共振和质谱分析数据表明，Ag-DNA 棒在溶液中和晶体中都能形成。
