Slovenian NMR Center, National Institute of Chemistry, SI-1000, Ljubljana, Slovenia.
Faculty of Chemistry and Chemical Technology, University of Ljubljana, SI-1000, Ljubljana, Slovenia.
Nat Commun. 2024 Sep 5;15(1):7763. doi: 10.1038/s41467-024-51876-8.
The challenge of transforming organized DNA structures into their metallized counterparts persists in the scientific field. In this context, utilizing DNA molecules modified with 7-deazapurine, provides a transformative solution. In this study, we present the solution structure of a DNA duplex that can be transformed into its metallized equivalent while retaining the natural base pairing arrangement through the creation of silver-modified Watson-Crick base pairs. Unlike previously documented X-ray structures, our research demonstrates the feasibility of preserving the intrinsic DNA self-assembly while incorporating Ag into the double helix, illustrating that the binding of silver does not disrupt the canonical base-pairing organization. Moreover, in our case, the uninterrupted Ag chain deviates from forming conventional straight linear chains; instead, it adheres to a helical arrangement dictated by the underlying DNA structure. This research challenges conventional assumptions and opens the door to precisely design structures based on the organization of highly stable Ag-DNA assemblies.
将组织化的 DNA 结构转化为其金属化对应物的挑战在科学界仍然存在。在这种情况下，利用经过 7-脱氮嘌呤修饰的 DNA 分子提供了一种变革性的解决方案。在这项研究中，我们提出了一种 DNA 双链体的溶液结构，通过创建银修饰的沃森-克里克碱基对，可以将其转化为其金属化的对应物，同时保留天然的碱基配对排列。与之前记录的 X 射线结构不同，我们的研究证明了在将银纳入双螺旋结构的同时保留内在 DNA 自组装的可行性，表明银的结合不会破坏规范的碱基配对组织。此外，在我们的情况下，不间断的 Ag 链偏离了形成传统直链的方式；相反，它遵循由基础 DNA 结构决定的螺旋排列。这项研究挑战了传统的假设，并为基于高度稳定的 Ag-DNA 组装的结构的精确设计开辟了道路。
