Safwat Osama, Elkateb Mona, Dowidar Karin, Salam Hala Abdel, El Meligy Omar
J Clin Pediatr Dent. 2018;42(1):11-20. doi: 10.17796/1053-4628-42.1.3. Epub 2017 Sep 22.
To assess the microbial effect of ozone gas on dentinal lesions in young permanent molars using the stepwise excavation.
An experimental, controlled clinical trial was performed. The sample included 80 immature first permanent molars, showing deep occlusal carious cavities that were indicated for stepwise excavation. Following first step of dentin excavation, the sample was divided into test (ozone gas) and control (calcium hydroxide (Ca(OH)) base material) groups. One half of the cases in each group were evaluated for microbiological changes after 6 months, and the other half after 12 months.
Mutans streptococci (MS), Lactobacilli, and Candida counts were significantly reduced immediately after ozone application in the test group (P ≤0.05). At the final assessment period, MS and Lactobacilli were significantly reduced in the test group (P ≤0.05). Meanwhile, the Candida counts were significantly reduced only in the test group of the 6 and 12 month-cases (P ≤0.05). Regarding the control group, the significant reduction in microbial count was observed with MS after 6 and 12 months (P ≤0.05). No significant differences were observed between test and control groups at different evaluation periods (P >0.05).
Ozone gas had a significant antimicrobial effect in deep class I carious lesions.
采用逐步窝洞预备法评估臭氧气体对年轻恒牙牙本质龋损的微生物学影响。
进行了一项实验性对照临床试验。样本包括80颗未成熟的第一恒磨牙，这些牙齿显示有深的咬合面龋洞，需进行逐步窝洞预备。在牙本质预备的第一步之后，将样本分为试验组（臭氧气体）和对照组（氢氧化钙（Ca(OH)）基材料）。每组中一半病例在6个月后评估微生物学变化，另一半在12个月后评估。
试验组在应用臭氧后，变形链球菌（MS）、乳酸杆菌和念珠菌计数立即显著降低（P≤0.05）。在最终评估期，试验组的MS和乳酸杆菌显著减少（P≤0.05）。同时，仅在6个月和12个月病例的试验组中念珠菌计数显著减少（P≤0.05）。关于对照组，在6个月和12个月时观察到MS的微生物计数显著降低（P≤0.05）。在不同评估期，试验组和对照组之间未观察到显著差异（P>0.05）。
臭氧气体对深I类龋损有显著的抗菌作用。
