Anhui Province Agricultural Products Processing Engineering Laboratory, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Agricultural Products Processing Engineering Laboratory, Anhui Agricultural University, Hefei 230036, China; Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
Int J Biol Macromol. 2018 Feb;107(Pt A):882-890. doi: 10.1016/j.ijbiomac.2017.09.062. Epub 2017 Sep 20.
The swollen culms of Zizania latifolia is a popular vegetable and traditional herbal medicine mainly consumed in some Asian countries. In our previous study, water extractable polysaccharide (ZLPs-W) and alkali extractable polysaccharide (ZLPs-A) was sequentially prepared from the swollen culms of Zizania latifolia. In vitro cell assay revealed that ZLPs-W without cytotoxicity had higher immunomodulatory activity than ZLPs-A. Therefore, in this study, ZLPs-W was further separated to three purified polysaccharides of ZLPs-W1, ZLPs-W2, and ZLPs-W3 by anion exchange chromatography and size exclusive chromatography in sequence. They were found to be homogeneous polysaccharides in β-type glycosidic linkage. ZLPs-W1 and ZLPs-W2 without triple helix conformation mainly contained Glc in molar percentage of 96.47% and 85.1%, respectively. ZLPs-W3 with triple helix conformation mainly consisted of Man, Rha, GalA, Glc, Gal and Ara in the molar ratio of 10.23:14.14:37.44:17.14:16.54:4.51. In addition, all the three purified polysaccharides could effectively enhanced the proliferation, phagocytosis, and nitric oxide production of murine macrophage RAW 264.7, suggesting that they possessed a potent immunostimulatory activity and could be developed as immunomodulator in medicine or functional food areas.
菰米膨胀的茎秆是一种受欢迎的蔬菜和传统草药，主要在一些亚洲国家消费。在我们之前的研究中，从菰米膨胀的茎秆中依次制备了水溶性多糖（ZLPs-W）和碱溶性多糖（ZLPs-A）。体外细胞试验表明，无细胞毒性的 ZLPs-W 具有比 ZLPs-A 更高的免疫调节活性。因此，在本研究中，ZLPs-W 通过阴离子交换色谱和尺寸排阻色谱进一步分离为三种纯化多糖 ZLPs-W1、ZLPs-W2 和 ZLPs-W3。它们被发现是具有β型糖苷键的均一多糖。无三螺旋构象的 ZLPs-W1 和 ZLPs-W2 主要含有摩尔百分比为 96.47%和 85.1%的 Glc。具有三螺旋构象的 ZLPs-W3 主要由 Man、Rha、GalA、Glc、Gal 和 Ara 组成，摩尔比为 10.23:14.14:37.44:17.14:16.54:4.51。此外，这三种纯化多糖都能有效增强小鼠巨噬细胞 RAW 264.7 的增殖、吞噬和一氧化氮产生，表明它们具有很强的免疫刺激活性，可作为医学或功能性食品领域的免疫调节剂。
