箭叶慈姑多糖的优化、表征、流变性研究及免疫活性
Optimization, characterization, rheological study and immune activities of polysaccharide from Sagittaria sagittifolia L.
机构信息
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
出版信息
Carbohydr Polym. 2020 Oct 15;246:116595. doi: 10.1016/j.carbpol.2020.116595. Epub 2020 Jun 11.
To improve the extraction efficiency of polysaccharides from Sagittaria sagittifolia L. (SPU) by ultrasonic assisted extraction (UAE), the optimal extraction conditions were optimized as follows: extraction temperature of 85℃, extraction time of 15 min and ratio of liquid to raw material 43 mL/g, under these conditions, the yield of SPU increased by about 168 % compared with hot water extraction (HWE). After separation and purification by DEAE-52 cellulose column and Sephadex G-50 column, the pure polysaccharide fraction (SPU70-W1) was obtained, and its structure, rheology and immune activity were analyzed. The results indicated that SPU70-W1 (7.70 kDa) contained mannose, glucose and galactose in the molar ratio of 2.06:93.58:4.36 with typical pseudoplasticity fluids behavior and possessed the backbone of →2,6)-α-D-Glcp-(1→, →3,6)-α-D-Glcp-(1→, α-D-Glcp-(1→ and 6)-α-D-Glcp-(1→. In addition, SPU70-W1 exhibited remarkable immunomodulatory activity. Thus, SPU70-W1 could contribute to the food, medicine, cosmetics as a functional additive.
为了提高箭叶茨菰（SPU）多糖的超声辅助提取（UAE）提取效率，优化了最佳提取条件，如下：提取温度 85℃，提取时间 15min，液料比 43mL/g，在此条件下，与热水提取（HWE）相比，SPU 的产率提高了约 168%。经 DEAE-52 纤维素柱和 Sephadex G-50 柱分离纯化，得到纯多糖级分（SPU70-W1），并对其结构、流变学和免疫活性进行了分析。结果表明，SPU70-W1（7.70kDa）中含有甘露糖、葡萄糖和半乳糖，摩尔比为 2.06:93.58:4.36，具有典型的假塑性流体行为，具有→2,6)-α-D-Glcp-(1→、→3,6)-α-D-Glcp-(1→、α-D-Glcp-(1→和 6)-α-D-Glcp-(1→的主链。此外，SPU70-W1 表现出显著的免疫调节活性。因此，SPU70-W1 可作为一种功能性添加剂，应用于食品、医药、化妆品等领域。
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