海参（Apostichopus japonicus）组织蛋白酶 D 的分子克隆与功能鉴定。
Molecular cloning and functional characterization of cathepsin D from sea cucumber Apostichopus japonicus.
机构信息
School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.
School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.
出版信息
Fish Shellfish Immunol. 2017 Nov;70:553-559. doi: 10.1016/j.fsi.2017.09.011. Epub 2017 Sep 20.
Cathepsin D (CTSD, EC 3.4.23.5) belongs to aspartic protease family, which is located in lysosomes and is distributed in diverse tissues and cells. CTSD has a wide variety of physiological functions, owing to its proteolytic activity in degradating proteins and peptides. In the current study, the full length cDNA of sea cucumber (Apostichopus japonicus) cathepsin D (AjCTSD) was firstly cloned, then the association between AjCTSD and sea cucumber autolysis was investigated. The full length cDNA of AjCTSD was 2896 bp, with an open reading frame (ORF) for 391 amino acids. AjCTSD was widely expressed in body wall, muscle and intestine; the expression level was the highest in intestine, followed by muscle and body wall. Compared to fresh tissues, AjCTSD expression levels were significantly increased in all examined autolytic tissues. The purified recombinant AjCTSD promoted the degradation of sea cucumber muscle. In conclusion, AjCTSD contributed to sea cucumber muscle autolysis.
组织蛋白酶 D（CTSD，EC 3.4.23.5）属于天冬氨酸蛋白酶家族，位于溶酶体中，分布于多种组织和细胞。由于其在降解蛋白质和肽方面的蛋白水解活性，CTSD 具有广泛的生理功能。在本研究中，首次克隆了海参（Apostichopus japonicus）组织蛋白酶 D（AjCTSD）的全长 cDNA，然后研究了 AjCTSD 与海参自溶之间的关系。AjCTSD 的全长 cDNA 为 2896bp，开放阅读框（ORF）为 391 个氨基酸。AjCTSD 在体壁、肌肉和肠道中广泛表达；在肠道中的表达水平最高，其次是肌肉和体壁。与新鲜组织相比，AjCTSD 在所有检测到的自溶组织中的表达水平均显著升高。纯化的重组 AjCTSD 促进了海参肌肉的降解。综上所述，AjCTSD 有助于海参肌肉自溶。
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