Benoit Matthieu P M H, Sosa Hernando
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Jack and Pearl Resnick Campus, 1300 Morris Park Avenue, Bronx, NY, 10461, USA.
Methods Mol Biol. 2018;1665:199-216. doi: 10.1007/978-1-4939-7271-5_11.
Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor proteins along cytoskeletal tracks.
单分子荧光偏振显微镜（smFPM）是一种能够监测单个标记蛋白结构域取向变化的技术。在此，我们描述了一种smFPM显微镜设置及实验方案，用于研究与运动蛋白沿细胞骨架轨道移动相关的构象变化。
