Ribes G, Gross R, Chenon D, Puech R, Loubatieres-Mariani M M
Faculté de Médecine, Laboratoire de Pharmacologie et Pharmacodynamie, C.N.R.S., Montpellier, France.
Pancreas. 1987;2(6):638-44. doi: 10.1097/00006676-198711000-00003.
This work was undertaken to study the effect of glucose on pancreaticoduodenal and peripheral venous somatostatin-like immunoreactivity (SLI) levels in dogs. Our experiments were performed in normal and alloxan diabetic dogs, conscious or anesthetized. The response of somatostatin was studied following intravenous (0.2 g/kg) or oral (1 g/kg) glucose administration. SLI levels were assayed in peripheral venous blood and in superior pancreaticoduodenal venous blood. An interplay of the cholinergic nervous system was challenged both after oral and intravenous glucose load by a prior administration of atropine sulfate (0.2 mg/kg i.v.). Our results show that (a) peripheral venous SLI levels do not reflect pancreatic D-cell activity in alloxan diabetic as in normal animals. (b) Increase of peripheral venous SLI level after oral glucose is under cholinergic nervous system control. (c) In alloxan diabetic dogs, the response of pancreaticoduodenal venous SLI to intravenous glucose was decreased, whereas peripheral SLI response to oral glucose was increased.
本研究旨在探讨葡萄糖对犬胰十二指肠及外周静脉中类生长抑素免疫反应性（SLI）水平的影响。我们在正常犬和四氧嘧啶糖尿病犬身上进行了实验，这些犬处于清醒或麻醉状态。在静脉注射（0.2 g/kg）或口服（1 g/kg）葡萄糖后，研究了生长抑素的反应。在外周静脉血和胰十二指肠上静脉血中检测SLI水平。在口服和静脉注射葡萄糖负荷前，预先给予硫酸阿托品（0.2 mg/kg静脉注射），以挑战胆碱能神经系统的相互作用。我们的结果表明：（a）与正常动物一样，外周静脉SLI水平不能反映四氧嘧啶糖尿病动物的胰腺D细胞活性。（b）口服葡萄糖后外周静脉SLI水平的升高受胆碱能神经系统控制。（c）在四氧嘧啶糖尿病犬中，胰十二指肠静脉SLI对静脉注射葡萄糖的反应降低，而外周SLI对口服葡萄糖的反应增加。
