Ribes G, Gross R, Chenon D, Loubatières-Mariani M M
Laboratoire de Pharmacologie et Pharmacodynamie, Unité Associée au CNRS n. 599, Institut de Biologie, Montpellier, France.
Acta Endocrinol (Copenh). 1988 Sep;119(1):43-50. doi: 10.1530/acta.0.1190043.
The effect of insulin on basal pancreaticoduodenal output of SRIH was investigated in vivo and compared in normal and alloxan diabetic dogs. The experiments were performed on anesthetized dogs having a T-shaped catheter inserted into the pancreaticoduodenal vein just at the exit of the pancreas for blood sampling. In normal dogs, an insulin infusion (1 IU/kg for 20 min) or an iv insulin injection (0.2 IU/kg over 30 sec) produced, before any change in glycemia, an immediate reduction of the venous pancreaticoduodenal output of SRIH. Then pancreaticoduodenal output of SRIH rose close to starting values and decreased again when blood glucose level became very low. In alloxan-diabetic dogs, insulin infusion (1 IU/kg for 20 min) also induced an immediate inhibitory effect on pancreaticoduodenal SRIH output; the effect was more transient and from 20 min, unlike in normal dogs, an increase in pancreaticoduodenal output of SRIH was observed. In conclusion, exogenous insulin induces an immediate reduction in pancreaticoduodenal SRIH secretion both in normal and diabetic dogs, independently of basal blood glucose level and before any change in glycemia. In contrast, the delayed effect is different: SRIH secretion is reduced in normal dogs, whereas it is enhanced in diabetic dogs.
在正常犬和四氧嘧啶糖尿病犬体内研究了胰岛素对胰腺十二指肠生长抑素（SRIH）基础分泌量的影响，并进行了比较。实验在麻醉犬身上进行，将一个T形导管插入胰腺十二指肠静脉出口处进行采血。在正常犬中，输注胰岛素（1 IU/kg，持续20分钟）或静脉注射胰岛素（0.2 IU/kg，30秒内推注），在血糖发生任何变化之前，均可立即降低胰腺十二指肠静脉SRIH分泌量。然后，当血糖水平变得非常低时，胰腺十二指肠SRIH分泌量接近起始值升高，随后再次下降。在四氧嘧啶糖尿病犬中，输注胰岛素（1 IU/kg，持续20分钟）也对胰腺十二指肠SRIH分泌产生立即抑制作用；与正常犬不同，该作用更短暂，从20分钟起，观察到胰腺十二指肠SRIH分泌量增加。总之，外源性胰岛素在正常犬和糖尿病犬中均可立即降低胰腺十二指肠SRIH分泌，这与基础血糖水平无关，且在血糖发生任何变化之前。相反，延迟效应有所不同：正常犬中SRIH分泌减少，而糖尿病犬中SRIH分泌增加。
