Oncology Surgical, The First Affiliated Hospital of Xi'an Medical University, Xi'an 710074, China.
School of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China; Biochemistry and Molecular Biology Department, Xi'an Medical University, Xi'an, Shaanxi 710021, China.
Microb Pathog. 2017 Nov;112:171-175. doi: 10.1016/j.micpath.2017.09.048. Epub 2017 Sep 21.
In recent years Epithelial Mesenchymal Transition (EMT) has been proposed as a mechanism indispensable to acquisition of metastatic properties by tumor cells. In this study we tested the ability of Ferrerol, a Chinese herb-derived compound to ablate the EMT in human lung squamous cell carcinoma cells. Human lung squamous cell carcinoma cells, Calu-1 were treated with various concentrations of Ferrerol for 24 h to examine its effect on their viability by the MTT assay. Only those concentrations which showed least effect on the viability of Calu-1 cells were further used to evaluate the expression of epithelial and mesenchymal markers by western blotting. Furthermore the effect of such concentrations on the migration and invasion of Calu-1 cells was determined by wound healing and transwell invasion assays respectively. The results demonstrated that Ferrerol treatment led to the downregulation of Slug and Zeb-1, transcriptional regulators of EMT with the concomitant increase and decrease in the expression of E-cadherin and vimentin respectively. These data were further supported by migration and invasion assays which demonstrated that Ferrerol treatment caused inhibited the migration and invasion of Calu-1 lung squamous cell carcinoma cells. Taken together, our results indicate that Ferrerol suppresses lung squamous cell carcinoma cell metastatic potential by modulating the expression of EMT proteins.
近年来，上皮间质转化（EMT）被认为是肿瘤细胞获得转移特性所必需的机制。在这项研究中，我们测试了 Ferrerol，一种源自中药的化合物，是否能够消除人肺鳞癌细胞中的 EMT。用人肺鳞癌细胞 Calu-1 用不同浓度的 Ferrerol 处理 24 小时，通过 MTT 测定法检测其对细胞活力的影响。只有那些对 Calu-1 细胞活力影响最小的浓度才进一步用于通过 Western blot 评估上皮和间充质标记物的表达。此外，通过划痕愈合和 Transwell 侵袭测定分别评估这些浓度对 Calu-1 细胞迁移和侵袭的影响。结果表明，Ferrerol 处理导致 EMT 的转录调节剂 Slug 和 Zeb-1 的下调，同时 E-钙粘蛋白和波形蛋白的表达分别增加和减少。这些数据通过迁移和侵袭测定进一步得到支持，该测定表明 Ferrerol 处理抑制了 Calu-1 肺鳞癌细胞的迁移和侵袭。总之，我们的结果表明 Ferrerol 通过调节 EMT 蛋白的表达抑制肺鳞癌细胞的转移潜力。
