Shao Lei, Li Huanjie, Chen Jian, Song Haibo, Zhang Yuzhu, Wu Fei, Wang Wenjuan, Zhang Wen, Wang Fang, Li Hui, Tang Dongqi
Center for Stem Cell & Regenerative Medicine, The Second Hospital of Shandong University, Jinan, 250012, PR China; Jinan Central Hospital Affiliated to Shandong University, Jinan, 250012, PR China.
Jinan Central Hospital Affiliated to Shandong University, Jinan, 250012, PR China.
Biochem Biophys Res Commun. 2017 Apr 8;485(3):598-605. doi: 10.1016/j.bbrc.2016.12.084. Epub 2016 Dec 14.
Irisin is involved in promoting metabolism, immune regulation, and affects chronic inflammation in many systemic diseases, including gastric cancer. However, the role of irisin in lung cancer is not well characterized. To determine whether irisin has a protective effect against lung cancer, we cultured A549 and NCI-H446 lung cancer cells and treated them with irisin. We detected the proliferation by MTT assay, and assessed the migration and invasion of the cells by scratch wound healing assay and Tran-swell assay. The expression levels of epithelial-to-mesenchymal transition (EMT) markers and the related signaling pathways were detected by western blot analysis. Meanwhile, an inhibitor of PI3K was used to investigate the effect of irsin. Finally, the expression of Snail was detected. We demonstrated that irisin inhibits the proliferation, migration, and invasion of lung cancer cells, and has a novel role in mediating the PI3K/AKT pathway in the cells. Irisin can reverse the activity of EMT and inhibit the expression of Snail via mediating the PI3K/AKT pathway, which is a key regulator of Snail. These results revealed that irisin inhibited EMT and reduced the invasion of lung cancer cells via the PI3K/AKT/Snail pathway.
鸢尾素参与促进新陈代谢、免疫调节，并影响包括胃癌在内的许多全身性疾病中的慢性炎症。然而，鸢尾素在肺癌中的作用尚未得到充分表征。为了确定鸢尾素是否对肺癌具有保护作用，我们培养了A549和NCI-H446肺癌细胞并用鸢尾素处理它们。我们通过MTT法检测增殖情况，并通过划痕伤口愈合试验和Transwell试验评估细胞的迁移和侵袭能力。通过蛋白质免疫印迹分析检测上皮-间质转化（EMT）标志物的表达水平及相关信号通路。同时，使用PI3K抑制剂来研究鸢尾素的作用。最后，检测Snail的表达。我们证明鸢尾素可抑制肺癌细胞的增殖、迁移和侵袭，并在介导细胞内PI3K/AKT信号通路中发挥新作用。鸢尾素可通过介导PI3K/AKT信号通路逆转EMT活性并抑制Snail的表达，而PI3K/AKT信号通路是Snail的关键调节因子。这些结果表明，鸢尾素通过PI3K/AKT/Snail信号通路抑制EMT并减少肺癌细胞的侵袭。
