Tsiftsoglou A S, Barrnett R J, Sartorelli A C
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6381-5. doi: 10.1073/pnas.76.12.6381.
Friend murine leukemia cells induced to undergo erythrocytic differentiation by dimethyl sulfoxide give rise to progeny resembling ortho- or polychromatic normoblasts, which usually do not complete the maturation process to yield forms analogous to erythrocytes. Treatment of these differentiated cells with cytochalasin B can lead to a high proportion (i.e., 80-85%) of enucleated cells in vitro. Nuclear extrusion in cells induced to differentiate by dimethyl sulfoxide and subsequently treated with cytochalasin B began within 24-36 hr of exposure to the antibiotic, with the appearance of a pre-enucleated stage in which the cell nucleus became pycnotic and eccentrically located. Maximum enucleation occurred after 72-96 hr of exposure to cytochalasin B and was significantly enhanced when dimethyl sulfoxide was included in the culture medium during the period of treatment with cytochalasin B. Enucleation appeared to take place only in differentiated progeny, because nondifferentiated cells treated with cytochalasin B alone yielded a population of multinucleated cells. The findings indicate that highly tumorigenic nondifferentiated Friend erythroleukemia cells can be converted in high yield to mature enucleated forms that are unable to proliferate in vitro.
经二甲基亚砜诱导进行红细胞分化的弗氏小鼠白血病细胞产生的子代类似于正成红细胞或多染性正成红细胞，这些子代通常无法完成成熟过程以产生类似于红细胞的形态。用细胞松弛素B处理这些分化细胞可在体外导致高比例（即80 - 85%）的去核细胞。经二甲基亚砜诱导分化并随后用细胞松弛素B处理的细胞，在接触该抗生素24 - 36小时内开始核挤出，出现一个前去核阶段，此时细胞核固缩并偏心定位。在接触细胞松弛素B 72 - 96小时后出现最大去核率，并且当在细胞松弛素B处理期间培养基中含有二甲基亚砜时，去核率显著提高。去核似乎仅发生在分化的子代中，因为单独用细胞松弛素B处理未分化细胞会产生一群多核细胞。这些发现表明，高度致瘤的未分化弗氏红白血病细胞可高产转化为体外无法增殖的成熟去核形式。
