Postgraduate Institution, Guangzhou Medical University, Guangzhou 510182, P.R. China.
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518000, P.R. China.
Mol Med Rep. 2017 Nov;16(5):7665-7672. doi: 10.3892/mmr.2017.7579. Epub 2017 Sep 21.
The present study aimed to investigate idiopathic adhesive capsulitis (frozen shoulder), to gain insights on its pathogenesis, diagnosis and therapeutic targets. Using RNA‑sequencing (seq), the present study investigated differentially expressed genes (DEGs) in five samples from five idiopathic adhesive capsulitis patients and two samples from two acromioclavicular dislocation patients, without idiopathic adhesive capsulitis. The DEGs were analyzed using the following tools: Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathways analysis and protein‑protein interaction analysis. A total of 188 DEGs were identified and it was observed that 150 of these were upregulated and 38 were downregulated. It was hypothesized that various nutrient associated proteins may be associated with idiopathic adhesive capsulitis. The Matrix metalloproteinase family of proteins (MMPs), may exhibit a key role in the formation of abnormal collagen cross‑links. Overall, the comprehensive and detailed information collected in the present study, regarding idiopathic adhesive capsulitis, may provide a foundation on which in‑depth follow‑up experiments may be based, aimed at identifying novel strategies for treatment of this disease.
本研究旨在探讨特发性粘连性肩关节囊炎（冻结肩）的发病机制、诊断和治疗靶点。本研究使用 RNA 测序（seq）技术，对 5 例特发性粘连性肩关节囊炎患者和 2 例无特发性粘连性肩关节囊炎的肩锁关节脱位患者的 5 个样本和 2 个样本中的差异表达基因（DEGs）进行了研究。使用以下工具对 DEGs 进行了分析：基因本体富集分析、京都基因与基因组百科全书通路分析和蛋白质-蛋白质相互作用分析。共鉴定出 188 个 DEGs，其中 150 个上调，38 个下调。研究假设各种与营养相关的蛋白质可能与特发性粘连性肩关节囊炎有关。基质金属蛋白酶家族（MMPs）的蛋白质可能在异常胶原交联的形成中发挥关键作用。综上所述，本研究中关于特发性粘连性肩关节囊炎的全面和详细的信息，可能为深入的后续实验提供基础，旨在确定治疗这种疾病的新策略。
