Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University , Shanghai, 200433, China.
Anal Chem. 2017 Oct 17;89(20):11151-11158. doi: 10.1021/acs.analchem.7b03708. Epub 2017 Oct 5.
Developing cost-effective approaches based on hydrophilic interaction liquid chromatography (HILIC) has been the main tendency for low-abundance glycopeptides capture before LC-MS/MS analysis. Carbohydrates with outstanding biocompatibility and hydrophilicity are ubiquitous in the kingdoms of animal and plant and could be a wonderful choice as functional groups for glycopeptides enrichment. In this work, glucose-6-phosphate, as one of the indispensable cogs in pivotal metabolic wheels of life, was chosen as functionalized groups to be grafted onto the surface of FeO microspheres via one-step surface fabrication strategy. The acquired hydrophilic FeO@G6P microspheres showed superior enrichment performance for glycopeptides with high sensitivity (0.5 fmol/μL) and high selectivity (1:100) and good repeatability (10 times at least). Furthermore, the FeO@G6P microspheres also exhibited enrichment ability for glycopeptides in different biosamples. A total of 243 glycopeptides assigned to 92 glycoproteins and 183 glycopeptides corresponding to 74 different glycoproteins was identified from merely 2 μL of serum and saliva, respectively.
基于亲水作用色谱（HILIC）开发具有成本效益的方法一直是 LC-MS/MS 分析前低丰度糖肽捕获的主要趋势。具有出色生物相容性和亲水性的碳水化合物在动植物王国中无处不在，它们可以作为糖肽富集的功能基团，是一个不错的选择。在这项工作中，葡萄糖-6-磷酸作为生命关键代谢轮不可或缺的齿轮之一，被选择作为功能化基团，通过一步表面制造策略接枝到 FeO 微球表面。所获得的亲水 FeO@G6P 微球对糖肽具有优异的富集性能，具有高灵敏度（0.5 fmol/μL）和高选择性（1:100）以及良好的重现性（至少 10 次）。此外，FeO@G6P 微球还表现出对不同生物样品中糖肽的富集能力。分别仅从 2 μL 血清和唾液中鉴定出 243 种糖肽，对应 92 种糖蛋白和 183 种糖肽，对应 74 种不同的糖蛋白。
