Department of Chemistry, The Fifth People's Hospital of Shanghai, Institutes of Biomedical Sciences, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China.
Department of Chemistry, The Fifth People's Hospital of Shanghai, Institutes of Biomedical Sciences, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China.
Anal Chim Acta. 2018 Sep 18;1024:84-92. doi: 10.1016/j.aca.2018.04.030. Epub 2018 Apr 21.
As one of the most important post-translational modifications (PTMs) of peptidome, glycopeptidome is closely related to serious disease, especially to cancer. In order to specifically discover and analyze glycopeptidome biomarkers for clinical diagnosis of cancer on early-stage, it is crucial to develop efficient technique to analyze low-abundance of endogenous glycopeptides in biological samples. In this report, a hydrophilic probe in mesoporous pore (denoted as FeO@mSiO@G6P) was designed and prepared. By taking advantage of the excellent hydrophilicity and size-exclusion ability, we applied FeO@mSiO@G6P to capture glycopeptides from both horseradish peroxidase (HRP) and immunoglobulin (IgG) digests successfully. Moreover, a total of 39 and 25 endogenous glycopeptides were identified from healthy saliva and gastric saliva, respectively, indicating the great potential of this probe for the exploration of glycopeptidome biomarkers.
作为肽组中最重要的翻译后修饰（PTMs）之一，糖肽组与严重疾病，尤其是癌症密切相关。为了专门发现和分析糖肽组生物标志物，用于癌症的早期临床诊断，开发用于分析生物样品中内源性低丰度糖肽的有效技术至关重要。在本报告中，设计并制备了介孔孔中的亲水探针（表示为 FeO@mSiO@G6P）。利用优异的亲水性和尺寸排阻能力，我们成功地将 FeO@mSiO@G6P 用于从辣根过氧化物酶（HRP）和免疫球蛋白（IgG）消化物中捕获糖肽。此外，分别从健康唾液和胃液中鉴定出 39 种和 25 种内源性糖肽，表明该探针在探索糖肽组生物标志物方面具有巨大的潜力。
