Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
Anal Chem. 2017 Feb 7;89(3):1764-1771. doi: 10.1021/acs.analchem.6b04054. Epub 2017 Jan 18.
Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as FeO@mSiO-IDA) were designed and synthesized as an outstanding enrichment platform for glycopeptide analysis. By taking advantage of their merits, such as large surface area, excellent hydrophilicity and unbiased affinity toward all types of glycopeptides, the FeO@mSiO-IDA nanomaterials were successfully applied to capture glycopeptides from complex samples. A total of 25 glycopeptides from horseradish peroxidase (HRP) digests and 33 glycopeptides from IgG were identified, respectively. Especially, as a result, 424 glycopeptides assigned to 140 glycoproteins were identified from only 2 μL human serum.
亲水相互作用色谱（HILIC）是糖蛋白质组学分析中一种重要的富集策略。在本报告中，设计并合成了亲水性磁性介孔硅材料（表示为 FeO@mSiO-IDA），作为用于糖肽分析的出色富集平台。利用其优点，例如大的表面积、出色的亲水性和对所有类型糖肽的无偏亲和力，FeO@mSiO-IDA 纳米材料成功地用于从复杂样品中捕获糖肽。分别从辣根过氧化物酶（HRP）消化物中鉴定出 25 个糖肽，从 IgG 中鉴定出 33 个糖肽。特别是，仅从 2 μL 人血清中就鉴定出 424 个糖肽，归属于 140 种糖蛋白。
